RP-HPLC法分析酢浆草不同部位的酸性组分  

Analysis of Several Acidic Components in Different Parts of Oxalis corymbosa by RP-HPLC

作　　者：赵建芬[1] 李妍[1] 关萍萍[1] 

机构地区：[1]肇庆学院化学化工学院,肇庆526061

出　　处：《食品工业》2017年第5期280-284,共5页The Food Industry

基　　金：广东省高等学校优秀青年教师培养计划资助项目[项目名称:肇庆特色食品加工和资源利用(Yq2013164)]

摘　　要：建立同时分析草酸、酒石酸、苹果酸和柠檬酸的RP-HPLC法。色谱柱为Agilent Eclipse XDB-C18(4.6mm×250 mm,5μm),流动相为0.01 mol/L KH_2PO_4水溶液-甲醇(95∶5,V/V),pH 2.5,流速为1.0 mL/min,检测波长为210 nm,柱温为室温,进样量20μL。在建立的色谱条件下,各酸性组分获得良好的分离,质量浓度与峰面积之间呈良好线性关系,相关系数r>0.999,草酸、酒石酸和苹果酸的检出限分别为0.000 3,0.000 4和0.000 6 mg/mL。把所建立的RP-HPLC法用于酢浆草不同部位乙醇提取物酸性组分的分析,结果表明:样品液中未检出柠檬酸,在茎叶中各酸性组分含量为苹果酸>草酸>酒石酸,而在根部中为苹果酸>酒石酸>草酸,酢浆草不同部位提取物酸性组分含量存在一定的差异,其中草酸含量的差异最显著。在0.010 6~0.492 mg/mL加标浓度范围内样品各酸性组分加标回收率在99.0%~105.9%之间,RSD≤1.7%。所建立的RP-HPLC法操作简便,精密度高,可靠,适用于酢浆草中酸性成分含量的测定。Establish a RP-HPLC technique for simultaneous analysis of oxalic acid, tartaric acid, malic acid and citric acid. Chromatographic column was Eclipse XDB-C18 （250 ramx4.6 mm, 5 μm）, mobile phase was 0.01 mol/L KH2PO4-methanol （95 ： 5, V/V）, pH 2.5, flow rate was 1.0 mL/min, detection wavelength was 210 nm, column temperature was room temperature, and injection volume was 20 μL. Under these chromatographic conditions, four acidic components were well separated, a good linear relationship between peak area and concentration was observed for each analyte with a correlation coefficient r〉 0.999, and the detection limits of oxalic acid, tartaric acid and malic acid were 0.000 3, 0.000 4 and 0.000 6 mg/mL, respectively. The RP-HPLC technique was applied to analyze acidic components in ethanol extract of different parts of Oxalis corymbosa. The results showed that citric acid was not detected in the sample, the content of acidic components in stem leaf was malic acid〉 oxalic acid〉tartaric acid, and malic acid〉 tartaric acid〉 oxalic acid in root, the contents of acidic components in stem leaf and root of Oxalis corymbosa were some differences, the content of oxalic acid was the most significant difference. In spiked concentration range of 0.010 6-0.492 mg/mL, the average spike recoveries of oxalic acid, tartaric acid and malic acid in sample were 99.0%-105.9%, with RSD〈 1.7%. This technique was simple, high precision and reliable. It was suitable for the determination of acidic comoonents in Oxalis corvmbosa.

关 键 词：酢浆草 茎叶和根部 RP-HPLC法 酸性组分 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]