响应面法优化机械力辅助提取千里光酚类  

Optimization of mechanically assisted extraction of phenolic compounds in Senecio scandens Buch.-Ham.by response surface methodology

作　　者：俞静波[1] 王英瑶[1] 苏为科[1] 

机构地区：[1]浙江工业大学绿色制药协同创新中心,浙江杭州310014

出　　处：《食品工业科技》2017年第10期257-264,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(21406201);2015浙江省博士后科研项目择优资助

摘　　要：以绿原酸和金丝桃苷为得率指标,考察机械球磨参数和提取参数,通过响应面法优化,获得机械力辅助提取千里光酚类的最佳工艺条件。结果表明:最佳提取工艺参数为填充率27.6%,转速308 r/min,球磨时间11.5 min。随后经单次水提10 min,绿原酸得率为(5.040±0.042)mg/g,金丝桃苷得率为(0.485±0.004)mg/g。与乙醇热回流和水煎法相比,机械力辅助提取法能在较低的温度,无需使用有机溶剂下即可快速高效地获得酚类化合物,同时大大提高金丝桃苷的得率,减少提取物中吡咯里西啶类生物碱残留。Mechanical parameters and extraction parameters were studied by using the yields of chlorogenic acid and hyperoside as indicators, which afforded the optimal mechanically-assisted extraction condition for phenolic compounds from Senecio Scandens Buch.-Ham. by further employing response surface methodology. The results showed that the optimal mechanical parameters were as follows：milling ball filling degree 27.6%, milling speed 308 r/min and milling time 11.5 min, followed by single extraction in water for 10 min to give chlorogenic acid and hyperoside in （5.040 Senecio Scandens Buch.- Ham. ; phenolic compounds; mechanicallyassisted extraction; response surface methodology0.042） mg/g and （0.485 Senecio Scandens Buch.- Ham. ; phenolic compounds; mechanicallyassisted extraction; response surface methodology0.004） mg/g yield, respectively.Compared with heat reflux extraction and decoction, mechanically-assisted extraction was more efficient for rapid extraction of phenolic compounds at low extraction temperature without using organic solvent. Besides, the yield of hyperoside was increased substantially with obvious decrease of the residual pyrrolizidine in the extracts.

关 键 词：千里光 酚类 机械球磨提取 响应面优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]