滑子蘑多糖的抗肿瘤、抑菌活性及保湿特性  

Antitumor and Antibacterial Activity,Moisturizing of Pholiota nameko Polysaccharides

作　　者：李铭[1] 李文香[1] 孙亚男[1] 胡欣蕾 于戈[1] 张欣[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东省应用真菌重点实验室,山东青岛266109

出　　处：《北方园艺》2017年第10期131-135,共5页Northern Horticulture

基　　金：山东省现代农业产业技术体系建设经费资助项目(SDAIT-07-07)

摘　　要：以滑子蘑菌丝体为试材,利用热水浸提法提取多糖,采用体外细胞试验、琼脂凹环法,检测了滑子蘑多糖的抗肿瘤、抑菌活性,并研究了多糖的保湿特性,以期为滑子蘑多糖的开发利用提供参考依据。结果表明:与空白试验组相比,滑子蘑多糖对小鼠巨噬细胞Ana-1的促增长率为48.65%,对胃癌细胞HGC-27的增殖抑制率为58.21%,对乳腺癌细胞BT-549的增殖抑制率为76.83%,对宫颈癌细胞Hela-229的增殖抑制率为63.57%;滑子蘑多糖对革兰氏阴性菌大肠杆菌、沙门氏菌及革兰氏阳性菌金黄色葡萄球菌均有抑制作用,且随着浓度的升高,抑制性增强;在30℃、RH 45%的恒温恒湿条件下,保湿性大小顺序为壳聚糖>透明质酸>山梨醇>滑子蘑多糖>甘油,在30℃、RH 80%的恒温恒湿条件下,保湿性大小顺序为透明质酸>山梨醇>壳聚糖>滑子蘑多糖>甘油,2种条件下滑子蘑多糖的保湿率都高于甘油,说明滑子蘑多糖有较好的保湿性。Pholiota nameko was used as material，polysaccharides （PNP） was obtained by hot water extraction method.Antitumor and antibacterial activities were tested by cell experiment in vitro and agar concave ring method，respectively.Moisturizing capacity was also tested in order to provide a theoretical basis for the development and utilization of PNP.The results showed that PNP promoted the growth of Ana-1cells in vitro，reproduction rate was 48.65%，inhibited the growth of HGC-27，BT-549，Hela-229 cells in vitro，inhibiting rate was 58.21%，76.83%,63.57% respectively comparing with the blank group.The PNP had inhibited effect on Gram-positive bacteria such as Staphylococcus aureus and gram-negative bacteria such as Escherichia coli and Salmonella,the high concentration of PNP had the significant effect.Under the condition of 30 ℃ and RH 45%，moisturizing effect was chitosan〉hyaluronic acid〉sorbol〉PNP〉glycerol，under the condition of 30 ℃ and RH 80%，moisturizing effect was hyaluronic acid〉sorbol〉chitosan〉PNP〉glycerol.The moisturizing effect of PNP was better than glycerol under the two conditions，PNP had well moisturizing effect.

关 键 词：滑子蘑多糖 抗肿瘤 抑菌性 保湿性 

分 类 号：S646.16[农业科学—蔬菜学]