蔓性千斤拔黄酮类成分提取及抗氧化活性研究  

Total flavonoids extract and antioxidant capacity of Flemingia philippinensis

作　　者：王岩[1] 高建伟[1] 王伟[1] 薄力颖 肖云鹏[1] 张燊[1] 杨延鹏[1] 李冠龙[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,齐齐哈尔161006

出　　处：《食品科技》2017年第5期199-203,共5页Food Science and Technology

基　　金：教育部留学归国科研启动基金项目(第50批);齐齐哈尔大学青年教师科研启动支持计划项目(2014k-M27);齐齐哈尔大学大学生创新创业项目(201510221079)

摘　　要：以蔓性千斤拔为研究对象,乙醇为提取溶剂,考察不同提取方式及提取条件对蔓性千斤拔总黄酮的提取率和含量影响,并测定提取物对DPPH和羟自由基抗氧化性能。结果表明,超声波提取方式获得的提取率为7.95%,黄酮含量为0.75 mg/mL,明显高于回流提取及常温提取方式。通过单因素试验获得了最佳单因素条件,进行正交试验,得出在提取时间为20 min、料液比为1:4g/mL、乙醇体积分数为80%、提取温度温度80℃最佳条件下,提取物的黄酮含量为0.92 mg/mL;提取物对DPPH的清除率与抗坏血酸相当,对羟自由基具有一定的清除效果。黄酮类化合物的含量与提取物的抗氧化性能密切相关。研究结果将为蔓性千斤拔的开发利用提供技术参考,并为进一步分离纯化蔓性千斤拔中的黄酮类化合物打下基础。The study focus on total flavonoids contained in Flemingia philippinensis. The effects of different extraction methods and extraction conditions on the extraction rate and content of total flavonoids were studied. The antioxidant activities of extract against DPPH and hydroxyl free radicals were also determined by using ethanol as the extraction solvent. The results showed using ultrasound method that the extraction rate was 7.95% and the fiavonoids content was 0.75 mg/mL, which was significantly higher than that of reflux extraction and room temperature extraction. The optimal conditions were obtained by single factor experiment. The optimum conditions were also obtained by orthogonal test. The results showed that when the extraction time was 20 min, the ratio of material to liquid was 1：4 g/mL, the volume fraction of ethanol was 80% and the extraction temperature was 80 ℃ obtain the best condition. Under the optimal conditions, the flavonoids content of the extract was 0.92 mg/mL; the scavenging rate of DPPH was similar to that of ascorbic acid, and it had certain scavenging effect on hydroxyl radical. The content of flavonoids is closely related to the antioxidant activity of the extract. This study will provide technical reference for the development and utilization of Flemingia philippinensis, and lay a foundation for the further separation and purification of flavonoids in Flemingia philippinensis.

关 键 词：蔓性千斤拔 提取物 总黄酮 抗氧化 

分 类 号：R284.2[医药卫生—中药学]