不同类型蛋白胨支持大肠菌群生长效果评价  

Evaluation on effect of different peptone media on growth of cultured califorms

作　　者：赵贵明[1] 赵勇胜[1] 葛秋慧 杨海荣[1] 陈颖[1] 

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]北京奥博星生物技术有限责任公司

出　　处：《中国公共卫生》2017年第5期841-844,共4页Chinese Journal of Public Health

基　　金：国家"十二五"科技支撑计划课题(2012BAD29B02);国家质检总局科技计划项目(2012IK197)

摘　　要：目的快速、准确评价不同类型蛋白胨支持大肠菌群的生长效果,为优化大肠菌群快速生长培养基选择适宜原材料。方法选择不同类型的蛋白胨和大肠菌群代表性菌株,应用全自动生长曲线测定仪进行浊度测量,用原材料再配制培养基,通过测定培养基支持大肠菌群的生长能力验证原材料筛选方法的可靠性。结果多价蛋白胨支持大肠菌群生长能力较好;大肠菌群快速生长的蛋白胨评估与配制培养基后的实验结果一致,即培养24 h后最大A值均为1.18。结论不同类型蛋白胨支持大肠菌群的生长能力存在差异,生长曲线测定仪可作为培养基原材料选择的有力工具。Objective To evaluate the effect of different peptone media on the growth of cultured coliforms for selecting appropriate materials used in medium for rapid culturing of coliforms. Methods Growth curves of representative coliform strains cultured with different types of peptone medium were obtained through analyzing logarithmic growth phase with Bioscreen. Results Polypeptone and bacto tryptose showed better effect on the growth of all 4 tested strains among 8 tested peptones,with the same maximum absorbance value of 1.18 after 24 hour＇s culture in both cultures with different peptones and prepared media with those peptones. Conclusion The effect of various peptones on the growth of coliforms is different and growth curve analysis could be used in the selection of medium material for coliforms culture.

关 键 词：大肠菌群 浊度测量 蛋白胨 生长评价 

分 类 号：Q-331[生物学]