响应面优化酶法酯交换催化合成中长链结构甘三酯  

Optimization of Lipozyme 435-catalyzed synthesis of medium-and long-chain triglycerides using response surface methodology

作　　者：陆继源 王小三[1] 金青哲[1] 王兴国[1] 

机构地区：[1]江南大学食品学院,江苏省食品安全与质量控制协同创新中心,江苏无锡214122

出　　处：《中国油脂》2017年第5期15-19,共5页China Oils and Fats

基　　金：江苏省自然科学基金--青年基金项目(BK20150137);政策引导类计划(产学研合作)--前瞻性联合研究项目(BY2016022-33)

摘　　要：采用响应面法对脂肪酶Lipozyme 435在无溶剂体系中催化中链甘三酯(MCT)和大豆油酯交换反应合成中长链甘三酯的反应条件进行优化,并采用超高压液相色谱-飞行时间质谱在正离子模式下对反应产物的甘三酯构型进行分析。结果表明:反应温度和反应时间对中长链甘三酯含量具有极显著性影响(P<0.01)。最佳反应条件为大豆油与MCT质量比60∶40,反应时间4.36 h,反应温度89℃,加酶量(以底物质量计)5.5%。在此条件下,中长链甘三酯含量达到84.15%。CyCyL、CyCyLn、LOCy、LPCy、OOCy和LOCa为反应产物的主要甘三酯构型。The synthesis conditions of medium-and long-chain triglycerides by Lipozyme 435-catalyzed interesterification of soybean oil and medium-chain triglycerides in a solvent-free system was optimized by response surface methodology.The triglycerides structure of the product was analyzed by ultra-performance liquid chromatography （UPLC） coupled with quadrupole time of flight mass spectrometry under positive ion model.The results showed that reaction temperature and reaction time had extremely significant influence on medium-and long-chain triglycerides content.The optimal reaction conditions were obtained as follows：mass ratio of soybean oil to medium-chain triglycerides 60∶ 40,reaction time 4.36 h,reaction temperature 89 ℃,and enzyme dosage 5.5% （based on the mass of substrate）.Under these conditions,medium-and long-chain triglycerides content was 84.15%.The main triglycerides in the products were CyCyL,CyCyLn,LOCy,LPCy,OOCy and LOCa.

关 键 词：中长链结构甘三酯 LIPOZYME 435 酯交换 响应面 甘三酯构型 

分 类 号：TQ641[化学工程—精细化工]