非转基因和转基因小麦中氨基酸含量的分析及比较  

Determination and Comparison of Amino Acids in Non-transgenic Wheat and Transgenic Wheat

作　　者：刘婷婷[1] 邢青斌 程家丽[1] 沈葹[1] 卓勤[1] 

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《中国食物与营养》2017年第4期56-58,共3页Food and Nutrition in China

基　　金：转基因生物新品种培育国家科技重大专项课题"转基因生物的食用和饲用安全评价技术"(项目编号:2014ZX08011-005;2016ZX08011-005)

摘　　要：目的:对小麦中氨基酸分析的水解方法进行研究,比较非转基因和转基因小麦中氨基酸的含量。方法:样品用含0.1%巯基乙酸的盐酸溶液水解提取,样液经定容、过滤、浓缩、复溶、过膜后,用氨基酸分析仪测定,外标法定量。结果:应用含巯基乙酸的盐酸水解液对小麦样品进行水解的同时有效防止了含硫氨基酸(蛋氨酸和半胱氨酸)的氧化,相对氧化水解方法节省前处理时间,方法的回收率为93%~99%,相对标准偏差小于2.4%。非转基因和转基因小麦样品的17种氨基酸分析,氨基酸含量不存在显著性差异。结论:该方法准确快速、重现性好,适用于小麦中氨基酸含量分析的检测要求。[Objective] A new method for the determination on 17 amino acids in wheat was established by amino acid analyzer （AAA）. The contents of the amino acids in the non-transgenic wheat and the transgenic wheat were compared. [Method] The samples were subjected to hydrolysis by the hydrochloric acid solution containing 0. 1% thioglycolic acid, and were carried out by AAA. The external stand- ard method was used for quantitative analysis. [Result] This hydrolysis pretreatment process could effectively prevent oxidation of sulfur-containing amino acids （methionine and cysteine） . The recoveries were 93% -99% , and the relative standard deviations were no more than 2. 4 %. There was no significant difference of the 17 amino acids contents in the non-transgenic wheat and the genetransfer wheat [Conclusion] The method was accurate, and had good repeatability which could meet the requirements for determination of 17 amino acids in wheat.

关 键 词：氨基酸 转基因小麦 非转基因小麦 氨基酸分析仪 

分 类 号：S512.1[农业科学—作物学] R151.3[医药卫生—营养与食品卫生学]