脱氧紫色杆菌素抑制结肠癌TH29细胞增殖及诱导凋亡作用与机制  

Effect of deoxyviolacein on inhibiting proliferation and inducing apoptosis of colon cancer HT29 cells

作　　者：张海燕[1,2] 孙勇[3,4] 夏惠[2] 王锋[2] 王少康[2] 邢新会[3] 孙桂菊[2] 彭景[1] 

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225000 [2]东南大学公共卫生学院营养与食品卫生系,教育部环境医学工程重点实验室,南京210009 [3]清华大学化学工程系,工业生物催化教育部重点实验室,北京100084 [4]中国肉类食品综合研究中心,北京100068

出　　处：《中国比较医学杂志》2017年第5期89-93,共5页Chinese Journal of Comparative Medicine

摘　　要：目的探讨脱氧紫色杆菌素(Deoxyviolacein)对人结肠癌HT29细胞增殖抑制和诱导凋亡及其可能机制。方法用不同浓度的脱氧紫色杆菌素处理HT29细胞,采用四甲基偶氮唑蓝(MTT)比色法,检测脱氧紫色杆菌素对HT29细胞的抑制率;流式细胞术检测细胞周期和凋亡;Western Blot法检测凋亡相关蛋白Bax、Bcl-2和Caspase9表达的变化。结果脱氧紫色杆菌素对HT29细胞有明显的增殖抑制作用;可提高G0/G1期细胞比例,降低S期细胞比例;细胞凋亡率升高,Bax和Caspase9蛋白表达升高,Bcl-2蛋白的表达降低。结论脱氧紫色杆菌素抑制结肠癌HT29细胞增殖并诱导凋亡,其凋亡机制可能作用于线粒体介导的内源性通路。Objective To evaluate the effect of deoxyviolacein on the apoptosis in HT29 cells and its possible mechanisms of anticancer effects.Methods In vitro,human colon cancer HT29 cells were treated by deoxyviolacein in a series of concertrations.The absorbance value was detected by means of MTT assay and growth inhibition rate was calculate.Cell cycle and apoptosis was assessed by flow cytometry.The expression of Bax,Bcl-2 and Caspase9 were analyzed by Western blotting.Results Deoxyviolacein could inhibit the growth of human colon cancer HT29 cells.The cells at period G0/G1 and the apoptosis rates were significantly increased,and the cells at period S were significantly decreased.The expression of Bax,Caspase9 were upregulated and Bcl-2 was downregulated by deoxyviolacein at 48 hours.Conclusions Deoxyviolacein could inhibit the growth and induce apoptpsis of human colon cancer HT29 cells.The mechanism may be associated with up-regulation of Bax and Caspase9 and down-regulation of Bcl-2 expression.

关 键 词：脱氧紫色杆菌素 HT29细胞 凋亡 

分 类 号：R-33[医药卫生]