产几丁质酶微生物的筛选、鉴定及酶学性质研究  

Isolation and Identification of Chitinase-producing Strains and Studies on the Enzymatic Properties of Chitinase

作　　者：连文浩[1] 林娟[1,2] 王国增[1,2] 叶秀云[1,2] 苏冰梅[1] Lian Wenhao Lin Juan Wang Guozeng Ye Xiuyun Su Bingmei(College of Biological Science and Technology, Fuzhou University, Fuzhou 350116 National Engineering Laboratory for High Efficient Enzyme Expression, Fuzhou 350002)

机构地区：[1]福州大学生物科学与工程学院,福州350116 [2]酶高效表达国家工程实验室,福州350002

出　　处：《中国食品学报》2017年第3期82-89,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家海洋局海洋公益性行业科研专项(201305015);福建省海洋高新产业发展专项(闽海洋高新[2013]20号)

摘　　要：从福建地区土壤中筛选获得一株产几丁质酶菌株B120,经16S rDNA鉴定为蜡样芽孢杆菌。对菌株B120的产酶培养基进行优化,得到优化的培养基配方:乳糖3.5%,蛋白胨1.0%,细粉几丁质0.2%,K_2HPO_40.07%,KH_2PO_40.03%,MgSO_40.05%,FeSO_4·H_2O 0.002%,NaCl 0.5%,初始pH 6.0。在此条件下发酵84 h酶活力可达603 U/L,比优化前(87 U/L)提高了5.93倍。对几丁质酶酶学性质的研究表明,该酶的最适反应pH 8.0,最适反应温度50℃;在30℃、pH 7.0~10.0条件下保温1h,相对酶活力保持在74%以上,是一种中温碱性几丁质酶。A chitinase-producing strain B120：was isolated from Fujian soil and identified as Bacillus cereus by 16S rDNA sequence analysis. The chitinase-producing media were optimized and the optimal medium formulations were ob tained and as follow： lactose 3.5%, peptone 1.0%, powdered chitin 0.2%, K2HPO4 0.07%, KH2PO4 0.03%, MgSO4 0.05%, FeSO4·H2O 0.002%, NaCl 0.5%, pH 6.0. Fermenting under these conditions for 84 h, chitinase activity could reach 603 U/L, 5.93 times higher than before optimization （87 U/L）. Furthermore, the chitinase properties were studied. The results showed that the optimum reaction pH and temperature were 8.0 and 50 ℃, respectively. This enzyme was stable under 30 ℃ and pH 7.0-10.0, and more than 74% of relative activity was retained after incubation for 1 h. It was a kind of mesothennal and alkaline chitinases.

关 键 词：几丁质酶 筛选 鉴定 蜡样芽孢杆菌 培养基优化 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]