响应面法优化海湾扇贝壳赖氨酸螯合钙的制备工艺  

Optimization of preparation of calcium-lysine chelate from bay scallop shell by response surface method

作　　者：李云姣 陈杨扬 侯宝岩 霍超[1] 杜佳峰[1] 桑亚新[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《食品工业科技》2017年第12期248-253,共6页Science and Technology of Food Industry

基　　金：河北省科技支撑计划科研专项(16227109D);国家公益性行业科研专项(201205031)

摘　　要：为提高废弃扇贝壳的利用率,对扇贝壳制备赖氨酸螯合钙的工艺进行研究。以海湾扇贝壳和L-赖氨酸为原料,在一定条件下制备赖氨酸螯合钙,考察了反应温度、时间、L-赖氨酸与Ca^(2+)摩尔比例和pH对螯合产率的影响。在单因素的基础上进行响应面实验,确定螯合工艺为:反应温度63.4℃,反应时间34.0 min,L-赖氨酸与Ca^(2+)摩尔比例2.1∶1,pH为8.4。在此最优条件下,获得的产品螯合产率为82.16%,螯合物中的钙含量为9.58%。傅里叶红外光谱显示钙离子与L-赖氨酸发生了螯合反应,形成了Ca-N键和Ca-O键,有赖氨酸螯合钙的生成。In order to increase utilization of waste bay scallop shell, the preparation technology of calcium- lysine chelate from scallop shell was studied.Calcium chelating calcium was prepared from scallop shell and L-lysine under certain conditions.The effects of reaction temperature, time, L-lysine and Ca^2＋ molar ratio and pH value on chelate yield were investigated to determine reasonable preparation process.Response surface test was can＇ied out on the basis of single factor experiment, and the chelating process was determined as follows-reaction temperature was 63.4 ℃ , reaction time was 34.0 min,the molar ratio of L-lysine and Ca^2＋ was 2.1：1 ,pH value was 8.4.Under the optimum conditions, the chelate yield of product was 82.16% , and the calcium content in chelate was about 9.58% .Fourier transform infrared spectroscopy showed that calcium ions and L-lysine chelate reaction occured,formed Ca-N bond and Ca-O bond,and produced calcium-lysine chelate.

关 键 词：海湾扇贝壳 L-赖氨酸螯合钙 工艺优化 响应面法 

分 类 号：TS255.4[轻工技术与工程—农产品加工及贮藏工程]