超声波辅助消解-紫外可见分光光度法测定大肉姜中的硒  

Determination of Selenium in Zingiber officinale by Ultrasonic Wave Assisted Digestion-UV Visible Spectrophotometry

作　　者：谢微[1] 陈秋娟[2] 何星存[1] 罗杨合[1] 朱东建 

机构地区：[1]贺州学院食品科学与工程技术研究院,广西贺州542899 [2]贺州学院化学与生物工程学院,广西贺州542899

出　　处：《中国调味品》2017年第6期122-127,共6页China Condiment

基　　金：广西高校科学技术研究项目(KY2015LX477);广西科学研究与技术开发计划课题(桂科合14251003);贺州学院食品工程硕士专业学位学科建设科学研究培育项目(2014SGZSKY12);贺州学院校级课题(2014ZC28)

摘　　要：以邻苯二胺为络合显色剂,超声波辅助大肉姜的消解及硒与邻苯二胺的络合、萃取过程,利用紫外可见分光光度法测定大肉姜中的硒含量。结果表明:体系测定波长为334nm,硒在0~2.08μg/mL含量范围内有良好的线性关系,相关系数为0.9999,样品的最佳消解条件:消解体系为HNO_3-HClO_4,体积比为4∶1,用量为18mL的混酸,超声辅助消解时间为30min,水浴温度为50℃,测得大肉姜中的硒含量为4.4425μg/g,加标回收率为97.79%~102.02%,消解效果好,结果令人满意。O-phenylendiamine is used as chelating agent, with ultrasonic wave assisting the digestion of Zingiber officinale, complexation and extraction process of Se-o-phenylenediamine are determined. The content of selenium in Zingiber officinale is determined by UV visible spectrophotometry. The results show that the wavelength is 334 nm. This method has a good linearship of selenium content in the range of 0-2. 08μg/mL, and the correlation coefficient is 0. 9999. The best conditions for samples＇ digestion are： digestion system of HNO3-HClO4, volume ratio of 4 ： 1, mixed acid dosage of 18 mL, ultrasonicassisted digestion time of 30 rain,water bath temperature of 50 ℃, the selenium content in Zingiber officinale of 4. 4425μg/g, the adding standard recovery rate of 97.79%-102.02%. The result of digestion is efficacious and satisfactory.

关 键 词：硒 大肉姜 超声波 紫外可见分光光度法 

分 类 号：TS207.4[轻工技术与工程—食品科学]