响应面法优化产右旋糖酐肠膜明串珠菌的发酵培养基  

Optimization of the Fermentation Medium of Leuconostoc Mesentoroides(CICC-21725) by Response Surface Methodology

作　　者：李媚[1] 曾平[1] 袁宇[1] 覃琴[1] 陈熠嘉 廖安平[1] 

机构地区：[1]广西民族大学化学化工学院广西多糖材料与改性重点实验室培育基地广西高校化学与生物转化过程新技术重点实验室,广西南宁530006

出　　处：《食品研究与开发》2017年第11期170-175,共6页Food Research and Development

基　　金：广西高校人才小高地建设创新团队项目(桂教人[2011]47);广西科技型中小企业技术创新资金(桂科转13109067);广西民族大学-广西化工研究院研究生联合培养基地专项资金(研究生联合培养机制改-2013年113000100030001)

摘　　要：为提高右旋糖酐的产率,对肠膜明串珠菌CICC-21725发酵培养基进行优化。在单因素试验的基础上,根据中心设计原理,采用响应面法确定培养基成分间交互作用及最优组成。结果表明:优化后各培养基成分为蔗糖80.0 g/L,蛋白质7.0 g/L,磷酸氢二钠1.5 g/L,磷酸二氢钾0.3 g/L,在p H7.1、25℃、150 r/min时发酵21 h,右旋糖酐产率可达92.79%,与单因素优化后的右旋糖酐产率相比,提高了7.2%。红外光谱及核磁共振分析表明,发酵产物为右旋糖酐。In order to improve the yield of dextran, the fermentation midium of Leuconostoc Mesenteroides CICC-21725 had been optimized. On the basis of single factor experiments, according to the principle of design center, the response surface method was adopted to determine the interaction between medium composition and the optimal composition of medium composition. The medium optimized results indicated when sucrose, pep- tone, disodium hydrogen phosphate and potassium dihydrogen phosphate were 80.0, 7.0, 1.5 g/L and 0.3 g/L, adjusted pH to 7.1, fermentation 21 h at 25 ℃ and 150 r/min, dextran yield could reach 92.79 %. The dextran yield increased 7.2 % compared with the best ruslt after single factor optimization. Infrared spectrum and nuclear magnetic resonance （NMR） analysis showed that the fermentation products was dextran. The dextran yield was also improved after the medium composition had optimized through the response surface method.

关 键 词：肠膜明串珠菌 右旋糖酐 培养基优化 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]