嗜热真菌β-葡萄糖苷酶基因克隆表达与调控的研究进展  

Advances on Gene Cloning, Expression and Regulation of β-glucosidase from Thermophilic Fungi

作　　者：来亚鹏 刘刚 王娟[1] 

机构地区：[1]深圳大学生命与海洋科学学院,深圳市微生物基因工程重点实验室,广东深圳518060 [2]深圳大学生命与海洋科学学院,深圳市海洋生物资源与生态环境重点实验室,广东深圳518060

出　　处：《纤维素科学与技术》2017年第2期69-76,共8页Journal of Cellulose Science and Technology

基　　金：深圳市科技计划基础研究项目(JYJC20140418091413587)

摘　　要：β-葡萄糖苷酶是一种糖苷水解酶,可水解纤维二糖生成两分子的葡萄糖。该酶在纤维素糖化水解过程中起关键性作用,是纤维素酶代谢途径中的限速酶。嗜热真菌因其极端的生长环境,是耐高温酶的主要来源,嗜热真菌来源的β-葡萄糖苷酶种类日益丰富。构建了含13种嗜热真菌β-葡萄糖苷酶的进化树,并对嗜热真菌中β-葡萄糖苷酶的来源、嗜热真菌β-葡萄糖苷酶基因的克隆与表达、及已知的β-葡萄糖苷酶调控因子进行了综述。Beta-glucosidase is a kind of glycoside hydrolase. Cellobiose can be hydrolyzed into two molecules of glucose by p-glucosidase. It has a crucial role in the hydrolysis of cellulose. At the same time, it is the rate-limiting enzyme in the metabolic pathway of cellulose. Thermophilic fungi are the main source of high temperature resistant enzymes because of their habitat. The types of β-glucosidases from thermophilic fungi are increasingly abundant. The evolutionary tree of 13 kinds of β-glucosidases from different thermophilicfungi was constructed. This paper focuses on the source of β-glucosidases in thermophilicfungi, its gene cloning, expression and regulation.

关 键 词：嗜热真菌 Β-葡萄糖苷酶 基因克隆与表达 基因调控 

分 类 号：Q933[生物学—微生物学]