Proteomic profiling of early degenerative retina of RCS rats  

作　　者：Zhi-Hong Zhu Yan Fu Chuan-Huang Weng Cong-Jian Zhao Zheng-Qin Yin Zhi-Hong Zhu;Yan Fu;Chuan-Huang Weng;Cong-Jian Zhao;Zheng-Qin Yin(Southwest Hospital/Southwest Eye Hospital,Third Military Medical University;Key Lab of Visual Damage and Regeneration & Restoration of Chongqing)

机构地区：[1]Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, China [2]Key Lab of Visual Damage and Regeneration ＆ Restoration of Chongqing, Chongqing 400038, China

出　　处：《International Journal of Ophthalmology(English edition)》2017年第6期878-889,共12页国际眼科杂志（英文版）

基　　金：Supported by the National Nature Science Foundation of China(No.81130017);National Basic Research Program of China(973 Program,No.2013CB967002)

摘　　要：AIM： To identify the underlying cellular and molecular changes in retinitis pigmentosa （RP）. METHODS： Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole - orbitrap MS/MS, to identify the significant differentially expressed proteins （DEPs）. Bioinformatics analyses, including Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway annotation and upstream regulatory analysis, were then performed on these retina proteins. Finally, a Western blotting experiment was carried out to verify the difference in the abundance of transcript factor E2F1. RESULTS： In this study, we identified a total of 2375 protein groups from the retinal protein samples of RCS rats and non-dystrophic congenic controls. Four hundred thirty-four significantly DEPs were selected by Student＇s t-test. Based on the results of the bioinformatics analysis, we identified mitochondrial dysfunction and transcription factor E2F1 as the key initiation factors in early retinal degenerative process. CONCLUSION： We showed that the mitochondrial dysfunction and the transcription factor E2F1 substantially contribute to the disease etiology of RP. The results provide a new potential therapeutic approach for this retinal degenerative disease.AIM： To identify the underlying cellular and molecular changes in retinitis pigmentosa （RP）. METHODS： Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole - orbitrap MS/MS, to identify the significant differentially expressed proteins （DEPs）. Bioinformatics analyses, including Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway annotation and upstream regulatory analysis, were then performed on these retina proteins. Finally, a Western blotting experiment was carried out to verify the difference in the abundance of transcript factor E2F1. RESULTS： In this study, we identified a total of 2375 protein groups from the retinal protein samples of RCS rats and non-dystrophic congenic controls. Four hundred thirty-four significantly DEPs were selected by Student＇s t-test. Based on the results of the bioinformatics analysis, we identified mitochondrial dysfunction and transcription factor E2F1 as the key initiation factors in early retinal degenerative process. CONCLUSION： We showed that the mitochondrial dysfunction and the transcription factor E2F1 substantially contribute to the disease etiology of RP. The results provide a new potential therapeutic approach for this retinal degenerative disease.

关 键 词：retinal degeneration PROTEOMICS MITOCHONDRION E2F1 MaxQuant RCS rat 

分 类 号：R774.1[医药卫生—眼科]