几种食用菌多糖的提取与抗氧化性研究  

Extraction,Purification and Antioxidant Activities of Several Polysaccharides from Edible and Medicinal Fungi

作　　者：魏华[1] 张娣[1] 陆玲[1] 陈福程 

机构地区：[1]南京师范大学生命科学学院江苏省微生物与功能基因组学重点实验室江苏省微生物资源产业化工程技术研究中心,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2017年第2期72-75,88,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省"六大人才高峰"第八批高层次人才项目;国家基础科学人才培养基金(J1103507);江苏省高校自然科学基金(16KJB180016)

摘　　要：采用热水浸提法分别提取香菇(Lentinus edodes)、木耳(Auricularia polytricha)、银耳(Tremella)、猴头菇(Hericium erinaceus)、茶树菇(Agrocybe chaxinggu)5种食药用菌子实体中的水溶性多糖,子实体粉末在一定的料水比下热水浸提,胰蛋白酶法脱杂蛋白,乙醇沉淀得到精多糖.并采用苯酚-硫酸法测其糖含量,DPPH(1,1-二苯基-2-三硝基苯肼)法检测其抗氧化性.结果显示,茶树菇的精多糖得率最高,为4.81%,香菇其次,木耳最低;含糖量猴头菇最高,为53.9%,木耳最低;5种食药用菌子实体多糖均具抗氧化性、DPPH清除作用,茶树菇最强,浓度为1.13 mg/m L时清除率为50%,香菇次之,银耳最弱.Water soluble polysaccharide were extracted by hot water from five kinds of edible and medicinal fungus- Lentinus edodes ,Auricularia polytricha ,Tremella ,Hericium erinaceus ,Agrocybe chaxinggu. First, sporophore powder were extracted by hot water in certain ratio of material to water. And, hybridprotein were removed by trypsase. Then, refined polysaccharide were obtained by ethanol precipitation. The sugar content was tested by phenol sulfuric acid method.The antioxidant activity was detected by the DPPH（ 1, l-two-2-three dinitrophenylhydrazine） method.

关 键 词：多糖 食用菌 分离纯化 总糖含量 抗氧化性 

分 类 号：Q539[生物学—生物化学]