双波长法测定山药中直链和支链淀粉含量  

Dual-wavelength Spectrophotometry Method for Measuring Amylase and Amylopectin Contents of Yam

作　　者：崔晋[1,2] 李建军[2] 马艳弘[2] 李亚辉[2] 魏建明 郝林[1] CUI Jin LI Jian-jun MA Yan-hong LI Ya-hui WEI Jian-ming HAO Lin(College of Food Science and Nutrition Engineering, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China Jiangsu Boda Biotechnology Ltd., Xuzhou 221723, Jiangsu, China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]江苏省农业科学院农产品加工研究所,江苏南京210014 [3]江苏博达生物科技有限公司,江苏徐州221723

出　　处：《食品研究与开发》2017年第13期150-154,共5页Food Research and Development

基　　金：江苏省农业自主创新基金项目(CX(16)1019)

摘　　要：使用双波长分光光度法测定不同品种山药中直链淀粉和支链淀粉的含量,根据双波长法的测定原理,确定山药直链及支链淀粉的测定波长和参比波长,直链淀粉分别为624 nm和410 nm,支链淀粉分别为538 nm和758 nm。试验结果表明,该测定方法标准曲线良好。直链淀粉在0~50μg/mL质量浓度范围内其碘复合物与吸光度呈线性关系(R^2=0.999 7),支链淀粉在0~250μg/mL质量浓度范围内其碘复合物与吸光度呈线性关系(R^2=0.998 4)。山药中直链淀粉含量为0.026 mg/mL,回收率在96.72%~98.73%之间;支链淀粉的含量为0.072 mg/mL,回收率在95.74%~97.87%之间,RSD均小于1.5%。Dual-wavelength spectrophotometry was applied to the determination of amylase and amylopectin content in yam. According to the principle of dual-wavelength method determination, determine the purple yam starch measure wavelength and reference wave length. Absorbencies at 624 nm and 410 nm were used for amy-lose content measurement, absorbencies at 538 nm and 758 nm were used for amylopectin content measure-ment. An excellent linear relationship between starch-iodine complex and absorbance was achieved in the range of 0-50μg/mL（R2=0.9997） for amylose and 0-250μg/mL（R^2=0.9984） for amylopectin. The content of amy-lase was 0.026 mg/mL and the average recovery was between 96.72%and 98.73%.The content of amylopectin was 0.072 mg/mL and the average recovery was between 95.74%and 97.87%, and the RSD were both less than 1.5%.

关 键 词：山药 双波长分光光度法 直链淀粉 支链淀粉 

分 类 号：O657.3[理学—分析化学] TS232[理学—化学]