鸢尾叶异黄酮超声波辅助提取工艺优化及抗氧化活性研究  

Optimization of Ultrasonic-Assisted Extraction Process and Antioxidant Activity of Isoflavones from Leaves of Iris tectorum Maxim

作　　者：毛涵[1,2] 牛蕾[2] 薛菲[2] 赵华龙[2] 汤新慧[2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]盐城师范学院江苏省滩涂生物资源与环境保护重点建设实验室,盐城224002

出　　处：《食品工业》2017年第7期148-152,共5页The Food Industry

基　　金：江苏省"青蓝工程"科技创新团队项目

摘　　要：采用超声波辅助法提取鸢尾(Iris tectorum Maxim)叶中异黄酮,运用正交试验优化其提取工艺,并运用自由基清除试验考察鸢尾叶异黄酮的抗氧化活性。研究结果表明,影响异黄酮提取率大小的因素依次为:乙醇体积分数>超声功率>超声时间>料液比,异黄酮提取的最佳工艺条件为:乙醇85%,料液比1︰25(g/mL),超声功率90W,超声时间50 min。此条件下鸢尾叶中异黄酮的得率为15.62 mg/g。此外,鸢尾叶异黄酮对DPPH及羟自由基均显示显著清除活性,表明其具有较强抗氧化活性。Ultrasonic-assisted extraction method was adopted to extract the isoflavones from leaves oflris tectorum Maxim, and an orthogonal design was performed to determine the optimum extract conditions. Also the in vitro antioxidant activity of the I. tectorum isoflavones was evaluated by using free radicals scavenging assays. The results showed that the factors affecting the isoflavones extraction rate descending order were as follows： ethanol concentration, ultrasonic power, ultrasonic time, and material to liquid ratio. The optimum extract conditions were 85% ethanol, material to liquid ratio 1 ： 25 （g/mL）, ultrasonic power 90 W, and ultrasonic time 50 min. Under these conditions, the yield of total isoflavones was 15.62 mg/g. It was also found that the isoflavones could significantly scavenge DPPH and hydroxyl radicals in a concentration-dependent manner, which indicated that I. tectorum leafisoflavones had strong in vitro antioxidant activity.

关 键 词：鸢尾 异黄酮 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]