Effect of Sophora flavescens alkaloid on aerobic vaginitis in gel form for local treatment  

作　　者：Wang Xiu Li Jianchun Hu Yuzhen Chen Wenyang Jin Yiguang 

机构地区：[1]Department of Pharmaceutical Sciences,Beijing Institute of Radiation Medi-cine,Beijing 100850,China [2]Department of Pharmacy,Beng-bu Medical College,Bengbu 233030,China [3]Department of Pharmacy,Bengbu Medical Col-lege,Bengbu 233030,China [4]Department of Pharmaceutical Sciences,Bei-jing Institute of Radiation Medicine,Beijing 100850,China

出　　处：《Journal of Traditional Chinese Medicine》2017年第3期314-320,共7页中医杂志（英文版）

摘　　要：OBJECTIVE: To investigate the effect of Sophora flavescens alkaloid(SFA) in gel form on aerobic vaginitis(AV) and the possible mechanism underlying the effects.METHODS: AV rat models were prepared by intravaginal inoculation of Escherichia coli and Staphylococcus aureus. SFA gel and placebo gel were intravaginally administered. In vivo antibacterial effects,vaginal microenvironment, vaginal smears, pathological tissues of vaginas, and retention of gel in the vaginal cavity were investigated.RESULTS: SFA gel had much higher antibacterial effect than placebo gel. SFA gel protected the vaginal mucosa from erosion of bacteria. At the same time,they inhibited the inflammatory responses, exhibiting little leukocytes and parabasal cells. Furthermore, the number of vaginal Lactobacilli remarkably increased following administration of SFA gel.However, the vaginal p H did not recover to thehealthy acidic levels after treatment due to the buffering effect of gel. The gel of a fluorescent agent,Cyanine 7, showed very long retention time in the vaginal cavity, up to more than 24 h, much longer than the solutions.CONCLUSION: The SFA gel is a promising medicine for local treatment of AV with the advantages of anti-bacteria, protection of vaginal mucosa, increase of Lactobacilli, and long retention time in the vaginal cavity.OBJECTIVE： To investigate the effect of Sophora flavescens alkaloid（SFA） in gel form on aerobic vaginitis（AV） and the possible mechanism underlying the effects.METHODS： AV rat models were prepared by intravaginal inoculation of Escherichia coli and Staphylococcus aureus. SFA gel and placebo gel were intravaginally administered. In vivo antibacterial effects,vaginal microenvironment, vaginal smears, pathological tissues of vaginas, and retention of gel in the vaginal cavity were investigated.RESULTS： SFA gel had much higher antibacterial effect than placebo gel. SFA gel protected the vaginal mucosa from erosion of bacteria. At the same time,they inhibited the inflammatory responses, exhibiting little leukocytes and parabasal cells. Furthermore, the number of vaginal Lactobacilli remarkably increased following administration of SFA gel.However, the vaginal p H did not recover to thehealthy acidic levels after treatment due to the buffering effect of gel. The gel of a fluorescent agent,Cyanine 7, showed very long retention time in the vaginal cavity, up to more than 24 h, much longer than the solutions.CONCLUSION： The SFA gel is a promising medicine for local treatment of AV with the advantages of anti-bacteria, protection of vaginal mucosa, increase of Lactobacilli, and long retention time in the vaginal cavity.

关 键 词：MATRINE Escherichia coli LACTOBACILLUS Administration  intravaginal MICROENVIRONMENT Staphylococcus aureus 

分 类 号：R285.5[医药卫生—中药学]