贵州产地火棘活性成分的聚类分析  

Cluster Analysis on Antioxidant Composition of Pyracantha fortuneana (Maxim.) Li in Different Growing Regions of Guizhou

作　　者：谢国芳[1,2] 周晓珊[1,2] 韦开红 罗桥兰 刘志刚[1,2] 

机构地区：[1]贵阳学院食品与制药工程学院,贵州贵阳550005 [2]贵州省果品加工工程技术研究中心,贵州贵阳550005

出　　处：《食品与生物技术学报》2017年第6期642-648,共7页Journal of Food Science and Biotechnology

基　　金：贵州省教育厅自然科学研究重点项目(黔教合KY字[2014]276);贵州省科技厅-贵阳市科技局-贵阳学院联合基金项目(黔科合LH字[2014]7179号)

摘　　要：为了解不同地区野生火棘果实的抗氧化活性成分差异,以贵州贵阳、思南、长顺、习水等地采集的新鲜野生果实为材料,通过测定不同产地火棘果实的营养成分和抗氧化活性,利用IBM SPSS Statistics 19软件进行组内显著性分析、各指标间相关性、不同产地营养成分的主成分分析和聚类分析。结果表明:贵州不同产地火棘果实中营养成分及抗氧化活性差异显著,其中鱼梁河果实中抗坏血酸、蛋白质质量分数最高(分别为AAE(7.35±0.08)mg/hg和BAE(10.48±0.30)mg/hg;花溪火棘果实中总酚和总黄酮含量最高(分别为GAE(218.14±0.70)mg/hg和RE(159.80±0.45)mg/hg);岩上总酸含量最高,柠檬酸(7.18±0.00)g/kg;种获乡还原糖质量分数4.91%±0.01%;花溪火棘提取物的总还原力最强,岩上火棘提取物对超氧自由基清除率的最强,中心村火棘提取物对过氧化氢清除率最强,总酚、总黄酮、蛋白质、总酸质量分数与总还原力和超氧自由基清除率有一定线性关系;通过主成分分析和聚类分析将不同产地火棘果实分为3类,揭示了贵州不同产地野生火棘果实营养成分和抗氧化活性地域分布的规律性和差异性。To know the differences in antioxidant composition of Pyracantha fortuneana（Maxim. ）Lifrom different growing regions of Guizhou Province,China,we investigated the nutritional composition and antioxidant activity of Pyracantha fortuneana （Maxim.）Li and evaluated the differences, correlation coefficient ,principal component and clustering analysis by using the software IBM SPSS Statistics 20. The results indicated that the differences among the nutritional composition and antioxidant activity from different growing regions are significant. The highest contents of total ascorbic acid and protein content were detected in Pyracantha fortuneana （Maxim.）Li fromYulianghe AAE （7.35±0.08 mg/hg and BAE（10.48±0.30） mg/hg, respectively）. The total polyphenol and total flavonoids of Pyracantha fortuneana （Maxim.） Li from Huaxi were highest GAE （218.14± 0.70） mg/hg and RE （ 159.80±0.45 ） mg/hg, respectively）. The total acidity of Pyracantha fortuneana （Maxim.）Li from Yanshang were highest,citric acid （7.18 ±0.00） g/kg. The reducing sugar of Pyracantha fortuneana （Maxim.）Li from Zhonghuoxiang were highest （4.91±0.01 ）%. The total reducing power of Pyracantha fortuneana （Maxim.）Li from Huaxi was the strongest,while Pyracantha fortuneana （Maxim.）Li from Yanshang possessed the highest superoxide radical scavenging activity and Pyracantha fortuneana（Maxim.）Li from Zhongxincun possessed the highest hydrogen peroxide scavenging activity. The contents of total polyphenol, total flavonoids, protein and total acidity highly correlated with total reducing power and superoxide radical scavenging activity. The Pytacantha fortuneana（Maxim. ）Li of different growing regions were classified into three groups by principal component and cluster analysis. The regularity and difference of Pyracantha fortuneana （Maxim.）Li from different growing regions in Guizhou was revealed by principal component and cluster analysis.

关 键 词：火棘 产地 营养成分 抗氧化活性 

分 类 号：Q946[生物学—植物学]