酶联免疫法检测婴幼儿配方奶粉中的维生素B_(12)  

Detection of Vitamin B_(12) In Infant Formula Milk Powder By Enzyme Linked Immunosorbent Assay

作　　者：李江[1,2] 綦艳[1,2] 田秀梅 张娟[1,2] 龙顺荣 严家俊 

机构地区：[1]广东产品质量监督检验研究院,佛山528300 [2]国家食品质量监督检验中心(广东),佛山528300 [3]北京华安麦科生物技术有限公司,北京102200

出　　处：《食品工业》2017年第8期250-252,共3页The Food Industry

摘　　要：建立酶联免疫法检测婴幼儿配方奶粉中维生素B_(12)的分析方法,该方法线性相关系数大于0.996,范围在3~243μg/kg。奶粉样本经60%甲醇-水提取,再做1︰4稀释,通过酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)测定样本中维生素B_(12)。加标浓度为20μg/kg,奶粉中维生素B_(12)的回收率在95%~118%;加标浓度0,3,6,20和50μg/kg,样本的加标回收率在87%~102%;方法检出限为3μg/kg;与高效液相色谱法检测结果的相对标准偏差小于10%。方法操作简便,准确度和灵敏度高,可适用于婴幼儿配方奶粉中维生素B_(12)的检测。To establish a method for the detection of vitamin B12 in infant formula milk powder by enzyme linked immunosorbent assay （ELISA）, the method＇s linear correlation coefficient greater than 0.996 in the range of 3-243 μg/kg. The milk powder samples were extracted with 60% methanol-water and processed with 1 ： 4 dilution, and then the content of VBl2 in samples was determined by ELISA. When the spiked concentrations was 20 μg/kg, the recoveries of VBI2 in milk powder were 95%-118%; When the spiked concentrations were 0, 3, 6, 20 and 50 μg/kg, the recoveries of VBl2 in milk powder were 87%-102%; The limit of detection was 3 μg/kg; Compared with the High Performance Liquid Chromatography （HPLC） method, the relative standard deviation of result was less than 10%. This method was simple, accurate and sensitive, which was suitable for the detection of VBt2 in infant formula milk powder.

关 键 词：酶联免疫法 维生素B12 婴幼儿配方奶粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]