海洋低温淀粉酶菌株的筛选、鉴定及酶学性质研究  

Isolation, identification and enzymatic property of a low-temperature amylase-producing strain from marine

作　　者：周新尚 逄飞 窦少华[1,2,3] 乔慧[1,2] 迟乃玉[1,2] ZHOU Xinshang PANG Fei DOU Shaohua QIAO Hui CHI Naiyu(School of Lifc Science and Biotechnology, Dalian University, Dalian 116622, China Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian 116622, China School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]辽宁省海洋微生物生物工程技术研究中心,辽宁大连116622 [3]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《中国酿造》2017年第8期57-61,共5页China Brewing

基　　金：国家高技术研究发展计划"863计划"项目(No.2014AA093512);辽宁省自然科学基金项目(No.2014020134)

摘　　要：以大连黄海海泥和海水为样品,采用稀释涂布平板透明圈法初筛、摇瓶发酵复筛,得到一株淀粉酶高产菌ZXS-5,测得酶活为6.25 U/mL。通过形态学、生理生化及16S rDNA序列鉴定,菌株ZXS-5为荧光假单胞菌(Pseudomonas fluorescens)。该酶的最适作用温度为25℃,酶的热稳定性相对较差;最适作用pH值为8.0,属于碱性酶,该酶在酸性条件下稳定性较差;Ba^(2+)、Cu^(2+)、乙二胺四乙酸(EDTA)对该酶抑制性较强,Fe^(2+)、Zn^(2+)、Mn^(2+)对该酶的活性影响不明显,Mg^(2+)、Na^+对该酶激活作用较弱,Ca^(2+)对该酶激活作用较强。Using sea mud and seawater from Dalian Yellow Sea as samples, a higher amylase-producing strain ZXS-5 was obtained by spread plate method preliminary screening and shake flask fermentation secondary screening, and the enzyme activity was 6.25 U/ml. Through morphological, physiological and biochemical characteristics and 16S rDNA sequence identification, strain ZXS-5 was identified as Pseudomonas fluorescens. The optimum temperature of the amylase was 25 ℃ and its thermostability was relatively poor. The optimum pH of the amylase was 8.0, and it was alkaline amylase, and the pH stability was weak in acidic condition. The amylase activity was strongly inhibited by Ba^2＋, Cu^2＋ and ethylene diamine tetraacetic acid （EDTA）. The effects of Fe^2＋, Zn^2＋ and Mn^2＋ on the amylase activity were not obvious. The amylase activity was slightly activated by Mg^2＋ and Na＋, strongly activated by Ca^2＋.

关 键 词：低温淀粉酶 鉴定 荧光假单胞菌 酶学性质 

分 类 号：Q93-331[生物学—微生物学]