白簕中性均一多糖对链脲佐菌素诱导的糖尿病小鼠降糖作用机制研究  

Study on hypoglycemic effect and the mechanism of a neutral homogeneous polysaccharide from Acanthopanax trifoliatus on streptozotocin-induced diabetic mice

作　　者：周露[1] 杨慧文[1] 程轩轩[1] 张旭红[1] 潘育方[1] 杨全[1] 

机构地区：[1]广东药科大学中药学院、国家中医药管理局岭南药材生产与开发重点研究室,广东广州510006

出　　处：《食品工业科技》2017年第17期288-291,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(81503217)

摘　　要：目的:探讨白簕中性均一多糖组分(ATP1-1)对链脲佐菌素(STZ)诱导的I型糖尿病小鼠的降糖作用及机制。方法:采用多次小剂量注射链脲佐菌素(MLD-STZ)的方法,建立C57BL/6小鼠I型糖尿病模型。成模小鼠随机分成高血糖模型组、二甲双胍组、ATP1-1高剂量组和ATP1-1低剂量组,以同周龄正常小鼠为正常对照组,每组10只,给药4周。于给药后检测小鼠空腹血糖值(FBG)以及肝糖原含量,采用RT-PCR的方法测定肝脏中葡萄糖激酶(GK)、葡萄糖6磷酸酶(G6Pase)、葡萄糖转运体2(GLUT2)mRNA表达水平。结果:与模型组相比,ATP1-1高、低剂量组的血糖均显著下降(p<0.05),其中40 mg·kg^(-1)ATP1-1给药组血糖抑制率为29.4%;两剂量组均可有效增加糖尿病小鼠肝糖原含量(p<0.05);RT-PCR结果显示,ATP1-1高、低剂量组GK mRNA、GLUT2 mRNA表达显著增加(p<0.01),G6Pase mRNA表达降低(p<0.05)。结论:ATP1-1可以有效降低糖尿病小鼠血糖,该作用机制与调节糖代谢中的关键酶及相关转运体有关。Objective：To investigate the hypoglycemic effect and mechanism of a neutral homogeneous polysaccharide from Acanthopanax trifoliatus （ L. ） Merr. （ ATP1 - 1 ） on type I diabetic mice induced by STZ.Methods ： Type I diabetic animal model of C57BL/6 mice was established by multiple intraperitoneal injection of low dose streptozotocin（MLD-STZ） .Diabetic mice were randomly divided into hyperglycemia group, positive control group of metformin, high-dose and low-dose groups of ATP1-1. Normal mice were used as blank control, 10 mice for each group.The above components were administered intragastrically for 4 weeks.Hepatic glycogen and fasting blood glucose were determined at the end of administration.The mRNA expression of GK, G6Pase, GLUT2 were also observed by RT-PCR assay. Results： Compared with hyperglycemia group, FBG levels of the drug groups（ high-dose and low-dose of ATP1- 1 ）were decreased after 4 weeks＇ administration （p 〈 0.05 ）, meanwhile, hepatic glycogen content of the drug groups were inereased（p 〈0.05 ）.The inhibition rate of blood glucose for 40 mg·kg^-1 dose group was 29.4% .RT-PCR results showed that ATP1-1 could increase the mRNA expressions of GK and GLUT2 （p 〈 0.01 ）, while reduce the mRNA expression of G6Pase（p 〈 0.05 ）.Conclusion： ATP1-1 could decrease the blood glucose of type 1 diabetic mice, regulation of glyeometabolism might be one of the mechanisms.

关 键 词：白勒 多糖 降血糖 RT—PCR 

分 类 号：TS201.4[轻工技术与工程—食品科学]