不同红茶中主要氨基酸含量及其对小鼠自主活动的影响研究  

Comparison of the main amino acids content in different black tea and its effect on locomotor activity of mice

作　　者：徐欢欢[1,2,3] 尹丹[1,2,3] 刘提提[1,2,3] 李兆鹏 纪昌中[5] 黄业伟[1,2,3] 

机构地区：[1]云南农业大学普洱茶学教育部重点实验室,云南昆明650201 [2]云南省茶深加工工程技术研究中心,云南昆明650201 [3]云南农业大学食品科学技术学院,云南昆明650201 [4]安康市林业局,陕西安康725000 [5]安康学院,陕西安康725000

出　　处：《食品工业科技》2017年第17期300-304,共5页Science and Technology of Food Industry

基　　金：云南省应用基础研究计划青年项目(2016FD026);云南省教育厅科学研究基金项目(2017YJS025)

摘　　要：通过高效液相色谱法(HPLC)测定不同红茶中γ-氨基丁酸(γ-aminobutyric acid,GABA)、L-茶氨酸和L-谷氨酸的含量,并通过小鼠自主活动测试仪比较不同主要氨基酸含量的红茶水提物对小鼠自主活动的影响。结果表明,不同红茶中GABA、L-茶氨酸和L-谷氨酸含量差别均较大,并且L-茶氨酸含量普遍高于GABA、L-谷氨酸含量。其中,GABA含量以高黎贡山为最高,汉水韵红茶次之,紫鹃红最低;L-茶氨酸和L-谷氨酸含量均以八仙红茶为最高,汉水韵红茶次之,印度大吉岭红茶最低。此外,动物实验结果显示,与主要氨基酸含量较低红茶组相比,主要氨基酸含量较高红茶组小鼠的自主活动次数显著减少(p<0.05)。以上结果提示,主要氨基酸含量较高的红茶对小鼠具有明显的镇静作用。The γ-aminobutyric acid （GABA）, L-theanine and L-glutamic acid contents in different black tea were determined by high-performance liquid chromatography（ HPLC ）, and the effects of black tea water extract at different main amino acids contents on the locomotor activity of mice were monitored using a mouse activity monitor system.The results showed that the contents of GABA, L-theanine and L-glutamic acid were different in the different black tea samples, and the content of L-theanine was higher than that of GABA and L-glutamic acid. Among them, the GABA content in Gaoligongshan was the highest,followed by Haishuiyun black tea, and Zijuanhong was the lowest;the L-theanine and L-glutamic acid contents in Baxian black tea were the highest, followed by Haishuiyun black tea, and India Darjeeling black tea were the lowest. Additionally, the result of animal experiment showed that the locomotor activity level in the black tea with higher main amino acids content group was significantly decreased compared with that in the black tea with lower main amino acids content group （p 〈0.05）.The above results suggested that black tea with a high content of main amino acids had a significant sedative effect on mice.

关 键 词：γ-氨基丁酸(GABA) L-茶氨酸 L-谷氨酸 高效液相色谱法(HPLC) 自主活动能力 

分 类 号：TS201.4[轻工技术与工程—食品科学]