响应面法优化猪血浆蛋白制备铁螯合肽的酶解工艺  

Optimization of Enzymatic Hydrolysis Process for Preparation of Fe-Chelating Peptides from Porcine Plasma Proteins by Response Surface Methodology

作　　者：殷俊峰[1] 张福生[1] 朱峰 郭俊 范翠玲 朱梅 朱林 赵敏 

机构地区：[1]安徽省农业科学院农产品加工研究所,安徽合肥230031 [2]安徽珠峰生物科技有限公司,安徽太和236600

出　　处：《安徽农业科学》2017年第23期84-88,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化响应面法对猪血浆蛋白酶解制备铁螯合多肽的工艺。[方法]应用响应面法对猪血浆蛋白酶解制备铁螯合多肽的工艺进行优化,在单因素试验的基础上,选择pH、水解温度、水解时间为影响因素,以水解度为指标,进行3因素3水平的Box-Behnken中心组合试验设计,采用响应面法分析3个因素对响应值的影响。[结果]响应面法优化猪血浆蛋白制备蛋白粉的最佳工艺条件为pH7.7,温度46.3℃,水解时间7.4 h;在此条件下,蛋白的水解度为35.52%,水解物螯合率为79.41%。[结论]该工艺可为猪血浆蛋白开发利用提供新的思路。[Objective] To optimize the preparation of iron chelate polypeptide by hydrolysis of porcine plasma protein by response surface methodology. [Method] Response surface methodology was used to optimize the preparation of iron chelate polypeptide from porcine plasma protein hydrolysates,on the basis of single factor test,pH,hydrolysis temperature and hydrolysis time were selected as influencing factors,and hydrolysis degree as index,the Box-Behnken central composite experimental design of 3 factors and 3 levels,used the response surface method to analyze the influence of 3 factors on the response value. [Result]In response to the optimized process of preparation of protein powder surface method for preparation of porcine plasma protein pH 7. 7,hydrolysis temperature 46. 3 ℃,hydrolysis time 7. 4 h,under this condition,hydrolysis degree of protein hydrolysate was 35. 52%,chelation rate was 79. 41%. [Conclusion] This process can provide new ideas for the development and utilization of pig plasma proteins.

关 键 词：响应面法 猪血蛋白 水解 水解度 

分 类 号：S879[农业科学—畜牧兽医]