利用酵母粉和硫酸铜提高杏鲍菇漆酶活性  

Enhancing Laccase Production in Pleurotus eryngii Induced by Yeast Extract and Copper

作　　者：祝嫦巍[1] 鲍广稳 黄顺[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《中国食品学报》2017年第6期110-116,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31100070);安徽省高校省级自然科学研究项目重点项目(KJ2012A062);安徽省自然科学基金项目(1408085MC41)

摘　　要：研究杏鲍菇产漆酶的诱导物以及漆酶的合成规律。将不同碳源、氮源、金属离子添加到PDB基础培养基中,所有试验条件中,酵母粉和铜离子对发酵液中的漆酶活性诱导作用较强,然而作用方式不同。添加酵母粉,在培养初期即可使发酵液中的漆酶活性迅速增强,培养第6天达最大值,之后漆酶活性逐渐降低;添加铜离子对培养初期发酵液中的漆酶活性影响较小,菌体进入对数生长中后期,发酵液中的漆酶活性迅速增强,培养至第10天漆酶活性最大,之后相对稳定。培养基中同时添加酵母粉和硫酸铜对发酵液中的漆酶活性有协同促进作用,发酵液中的漆酶活性呈直线上升趋势,培养15 d,发酵液中的漆酶活性达(6 680.7±417.9)U/m L,是对照组最大漆酶活性的50倍。The inducers of laccase produced by P. eryngii and the regulation of laccase production were investigated in this paper. Different carbon sources, nitrogen sources and metal ions were added to the PDB basal medium, yeast extract and copper were good inducers among the tested substances, while acted differently. Laccase activity was increased greatly at the early stage of cultivation in the presence of yeast extract compared with the control. The highest laccase activity was obtained at the 6thday of cultivation in the presence of 1% yeast extract, and decreased gradually after that. Copper promoted laccase synthesis much greater in the mid-late of the logarithmic growth phase than that of in the early phase of cultivation. The highest laccase activity was obtained at the 10 thday of cultivation by adding 2mmol/L Cu2＋in the medium, and laccase activity was relatively stable after that. There existed a positive synergistic effect of yeast extract and copper on the laccase activity of P. eryngii. The laccase activity was increased greatly when yeast extract and copper were added into the medium simultaneously, laccase activity reached to（6 680.7±417.9） U/m L after 15 days of cultivation, 50 times of the highest laccase activity in the control group.

关 键 词：漆酶 杏鲍菇 酵母粉 硫酸铜 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]