乳酸菌生物学特性与其拮抗空肠弯曲杆菌在小鼠肠道定植能力的分析  

Analysis on biological characteristics of lactic acid bacteria and its antagonistic activity against Campylobacter jejuni colonization in mice

作　　者：王刚[1] 贺禹丰 陈晓华[2] 赵建新[1] 张灏[1] 陈卫[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]衡阳师范学院生命科学与环境学院,湖南衡阳421008

出　　处：《食品与发酵工业》2017年第8期73-80,共8页Food and Fermentation Industries

基　　金：国家自然科学基金-青年科学基金项目(31601444;31301407)

摘　　要：采用3周龄C57BL/6小鼠建立了空肠弯曲杆菌感染模型,评价了8株在体外具有抑制空肠弯曲杆菌生长能力的乳酸菌在体内拮抗空肠弯曲杆菌定植的能力,同时对该8株菌生长速率、产酸能力、生物膜形成能力、黏附能力等生物学特性进行了测定,通过主成分分析对8株乳酸菌生物学特性的评价以及皮尔森相关性系数对乳酸菌生物学特性及其在体内拮抗空肠弯曲杆菌定植能力相关性的分析,发现8株乳酸菌种间生物学特性差异明显大于种内生物学特性差异,且乳酸菌在小鼠体内拮抗空肠弯曲杆菌定植的能力与其细胞黏附能力呈正相关。具有最高黏附指数的植物乳杆菌N8、N9可有效清除模型小鼠肠道内的空肠弯曲杆菌,对保护人体免受空肠弯曲杆菌感染具有潜在的应用价值,同时也为具有拮抗致病菌能力的益生菌的筛选提供了理论依据。In this study, 3-week-old C57BL / 6 mice were used to establish a infection model by Campylobacter jejuni. 8 strains of lactic acid bacteria capable of inhibiting the growth of Campylobacter jejuniin vitro were engaged to estimate the antagonistic activity against Campylobacter jejuni colonization in vivo. Meanwhile, the biological characteristics such as the growth rate, acid production ability, biofilm formation ability and adhesion ability of the 8 strains were also measured. It was found that the differences of biological characteristics inter-species of 8 strains were greater than that of biological characteristics intra-species. Furthermore, the ability of lactic acid bacteria to antagonize Campylobacter jejuni colonization in mice was positively correlated with the adhesion ability of bacteria. Lactobacillus plantarum N8 and N9 with the highest adhesion index could effectively remove Campylobacter jejuni in the intestine of model mice. These two strains have the potential application value to prevent the human body from Campylobacter jejuni infection. This study also provides a theoretical basis for the screening of probiotics with antagonistic ability against pathogens.

关 键 词：乳酸菌 空肠弯曲杆菌 拮抗 黏附 小鼠感染模型 

分 类 号：TS201.3[轻工技术与工程—食品科学]