川陈皮素纳米结构脂质载体的制备及稳定性评价  

Preparation and stability evaluation for nobiletin nanostructured lipid carriers

作　　者：黄威[1,2] 窦华亭[1] 吴厚玖[1] 王华[1] 孙志高[1] 马亚琴[1] 黄林华[1] 郭莉[1] 王珺[1] 黄学根[1] 

机构地区：[1]西南大学柑桔研究所食品科学学院,重庆400712 [2]重庆文理学院林学与生命科学学院,重庆402160

出　　处：《食品与发酵工业》2017年第8期169-176,共8页Food and Fermentation Industries

基　　金：公益性行业(农业)科研专项(201303076-07);重庆市2016年基础科学与前沿技术研究一般项目(cstc2016jcyj A-0552)

摘　　要：纳米结构脂质载体(nanostructured lipid carrier,NLC)是一种新型的纳米脂质载体,将其搭载活性物质已经广泛应用于食品的多个相关领域。川陈皮素(nobiletin,NOB)是柑橘加工皮渣中提取的一种多甲氧基黄酮类化合物,具有多种生理活性,将其制备成纳米结构脂质载体可提高其溶解度、改善其分散性及生物利用率等。采用高压均质法制备了川陈皮素纳米结构脂质载体(NOB-NLC),运用单因素试验考察了脂质用量、乳化剂用量和乳化剂比例对NOB-NLC质量的影响。采用响应面设计优化了制备条件,确定的最适宜制备方案为脂质用量752.20 mg,乳化剂用量1 072.41 mg和乳化剂比例1.23(卵磷脂与泊洛沙姆188的质量比值),依据最适宜处方制备的NOB-NLC平均粒径为(132.63±7.28)nm,多分散指数为(0.253±0.015),Zeta电位为(-38.1±2.17)m V,包封率为(80.25±3.66)%。扫描电子显微镜显示所制备NOB-NLC呈球形或类球形,且大小具有良好的均一度。针对食品体系的应用,探究了贮藏条件对NOB-NLC稳定性的影响,结果表明在温度为25℃且pH为3.50~7.50的条件下贮藏40 d时,其粒径变化小,分散体系稳定性好,可应用于pH值为3.50~7.50的食品体系当中。Nanostructured Lipid Carriers （NLC） are a new lipid nanoparticle system and are wildly used in many fields of food science and process. Nobiletin, a citrus polymethoxylated flavone is extracted from citrus fruit peels,and has many health benefits. Nanostructured NLC can increase its water-solubility and bioavailability. High pressure homogenization was used to prepare nobiletin nanostructured lipid carrier （NOB-NLC）. Single factor design was chosen to study the influence of the amount of lipid, emulsifier and emulsifier ratio on the quality of NOB-NLC. Response surface design was used to optimize the preparation parameters. The optimal condition was ： 752.20 mg lipid,1072.41 mg emulsifier and 1.25 drug-lipid ratio. NLC -NOB exhibited spherical shape with diameter of （132.65 ± 7.28 ） nm, polydispersity index （0. 253 ± 0. 015 ）, zeta potential （ - 38.1 ± 2.17 ） mV and an entrapment efficiency （80.25 ± 3.66） %. The scanning electron microscope showed that the NOB-NLC were spherical shape. The influence of storage conditions on the stability of NOB-NLC was studied. The results indicated that NOB-NLC was stable after storage in dark at 25℃ ,pH 3.5 - 7.5 for 40 d, and the particle size is relatively consistent. It is available to the food systems with pH range of 3.50 - 7.50.

关 键 词：纳米结构脂质载体 川陈皮素 Box-Behnken试验 表征 稳定性 

分 类 号：TS202.3[轻工技术与工程—食品科学]