产水解银杏黄酮苷的β-葡萄糖苷酶菌株的筛选及鉴定  

Screening and identification of β-glucosidase-producing strain with hydrolysis of Ginkgo biloba flavonoid glycoside

作　　者：朱啟会 高泽鑫 何腊平 高冰 李翠芹[4] 熊江 张富豪 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州省农畜产品贮藏与加工重点实验室,贵州贵阳550025 [3]湖北工业大学生物工程与食品学院,湖北武汉430068 [4]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《中国酿造》2017年第9期102-106,共5页China Brewing

基　　金：国家自然科学基金(31160002);黔科合重大专项字[2015]6004-5号;黔科合支撑[2016]2580号

摘　　要：以银杏提取物为唯一碳源,利用栀子苷-谷氨酸钠显色法,从贵州银杏树土壤样品中分离产水解银杏黄酮苷的β-葡萄糖苷酶菌株。采用3,5-二硝基水杨酸(DNS)法测定β-葡萄糖苷酶酶活。得到一株酶活为1.03 U/m L的菌株NY-13,并且该菌株能水解芦丁生成槲皮素。经生理生化特征及16S r RNA分子生物学鉴定,菌株NY-13为解鸟氨酸柔武氏菌(Raoultella ornithinolytica)。Using the Ginkgo biloba extract as the sole carbon source, by the geniposide-sodium glutamate color method, the 13-glucosidase-producing strain which hydrolyzed Ginkgo biloba flavonoid glycoside was screened from Guizhou Ginkgo biloba trees soil samples. The β-glucosidase activity was determined by 3,5-dinitrosalicylic acid （DNS）. A strain NY-13 with 1.03 U/ml of β-glucosidase activity was obtained, and the strain could hy- drolyze rutin to produce quercetin. Through physiological and biochemical characteristics and 16S rRNA molecular biological identification, strain NY-13 was identified as Raoultella ornithinolytica.

关 键 词：Β-葡萄糖苷酶 银杏黄酮苷 筛选鉴定 解鸟氨酸柔武氏菌 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]