响应面法优化铜绿假单胞菌产鼠李糖脂发酵培养基的研究  

Optimization of fermentation medium components for rhamnolipid production by Pseudomonas aeruginosa with response surface methodology

作　　者：巩志金 刘胜格 梁鑫鑫 魏贝贝 梁光杰 杨革[1] 刘金锋[1] 车程川[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273100

出　　处：《中国酿造》2017年第9期127-131,共5页China Brewing

基　　金：国家级“本科教学工程”专业综合改革试点项目(ZG0293);高校应用型人才培养专业发展支持计划(鲁教高字[2015]5号);曲阜师范大学科技计划(xkj201610)

摘　　要：该研究对铜绿假单胞菌(Pseudomonas aeruginosa)SKY01产鼠李糖脂的发酵培养基组分进行了优化。采用单因素试验确定豆油、硝酸钠及微量元素的添加量。在单因素试验的基础上,采用响应面试验设计进行培养基组分优化。结果表明,最佳发酵培养基配方为豆油80 g/L、硝酸钠4 g/L、微量元素8.5‰。在此最佳培养基组分条件下,鼠李糖脂的产量达到45.34 g/L,比优化前的产量39.62 g/L提高了14.43%。The fermentation medium components for rhamnolipid production by Pseudomonas aeruginosa SKY01 were optimized in this paper. The additions of soybean oil, NaNO3 and trace elements were investigated by single factor experiments. Based on single factor experiments, response surface methodology were applied to optimize medium components. Results showed that the optimum fermentation medium formula were soybean oil 80 g/L, NaNO3 4 g/L and trace elements 8.5%o. Under these conditions, rhamnolipid yield was up to 45.34 g/L, which was 14.43% higher than that of before optimization （39.62 g/L）.

关 键 词：铜绿假单胞菌 鼠李糖脂 响应面法 培养基组分优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]