核酸适配体生物传感技术在食品污染物检测中的应用  

Applications of aptamer-based biosensing technology in the detection of food contaminants

作　　者：梁刚[1] 满燕[1] 李安[1] 潘立刚[1] LIANG Gang MAN Yan LI An PAN Li-Gang(Beijing Academy of Agriculture and Forestry Science, Beij＇ing Research Center for Agricultural Standards and Testing, Risk Assessment Lab for Agro-products（Beijing）, Ministry of Agriculture, Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China)

机构地区：[1]北京市农林科学院,北京农业质量标准与检测技术研究中心,农业部农产品质量安全风险评估实验室(北京),农产品产地环境监测北京市重点实验室,北京100097

出　　处：《食品安全质量检测学报》2017年第8期2936-2942,共7页Journal of Food Safety and Quality

基　　金：北京市农林科学院院创新能力建设专项(KJCX20170420);国家农产品质量安全风险评估重大专项子课题(GJFP201701403)~~

摘　　要：核酸适配体生物传感技术是近些年发展起来的一种新的检测技术,具有靶向性强、灵敏度高、价格低廉等优点,被广泛应用于医药工业、生物医学、环境分析、食品分析等领域。其中,在食品分析领域主要体现在农残、重金属、食品添加剂以及生物毒素等检测方面。本文首先介绍了核酸适配体的概念、筛选方法、核酸适配体传感器优点,重点从传感器的设计、传感检测原理、检测性能等方面综述了核酸适配体生物传感器在Pb^(2+)等食品污染物检测中的应用;最后对核酸适配体生物传感器的发展趋势进行了展望。Aptamer-based biosensing technology is a new detecting technique in recent years with the advantages of good target ability, high sensitivity and low cost, etc, which has been widely applied in the pharmaceutical industry, biology and medicine, environmental analysis and food analysis. For food analysis, aptamer-based biosensing technology is mainly used for detection of pesticide residues, heavy metals, food additives and biotoxin. This paper introduced the concept of aptamer, screening methods and the advantages of aptamer sensor firstly, and then emphatically reviewed the applications of aptamer sensor for the detection of food contaminants of Pb2＋ and pesticide residues from the points of conception of the sensor, sensening principle and detection performance. Finally, the development trends of the aptamer sensor in the future were prospected.

关 键 词：食品安全 适体 生物传感器 重金属 农药 

分 类 号：TS207.5[轻工技术与工程—食品科学]