湘玉竹多糖的体外抗氧化活性研究  

In vitro antioxidative effects of Polygonatum odoratum polysaccharides

作　　者：李化强 吴菲菲[1,2,3,4] 龙艳珍 赵良忠[1,2] 徐永平 李淑英 LI Hua-Qiang WU Fei-Fei LONG Yan-Zhen ZHAO Liang-Zhong XU Yong-Ping LI Shu-Ying(College of Food and Chemistry Engineering, Shaoyang University, Shaoyang 422000, China Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing, Shaoyang 422000, China School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd., Dalian 116620, China)

机构地区：[1]邵阳学院食品与化学工程学院,邵阳422000 [2]湖南省果蔬清洁加工工程技术研究中心,邵阳422000 [3]大连理工大学生命科学与生物技术学院,大连116024 [4]大连赛姆生物工程技术有限公司博士后科研工作站,大连116620

出　　处：《食品安全质量检测学报》2017年第8期3062-3067,共6页Journal of Food Safety and Quality

基　　金：国家科技部863计划项目(2013AA102805-03);湖南省果蔬清洁加工工程技术研究中心项目(2015TP2022);湖南省自科基金青年项目(2017JJ3284);湖南省教育厅优秀青年项目(17B241);湖南省教育厅一般项目(17C1433)~~

摘　　要：目的探索湘玉竹多糖的体外抗氧化效果。方法从湘玉竹中超声提取多糖,苯酚-硫酸法测定多糖含量。分别研究湘玉竹粗多糖和精制多糖对DPPH、O_2^-·、·OH和NO_2^-的清除作用,并与VC阳性对照进行比较,考察湘玉竹多糖的抗氧化效果。结果湘玉竹粗多糖中多糖含量为6.05%,精制多糖中多糖含量为13.26%。在试验浓度范围内,湘玉竹粗多糖和精制多糖对DPPH、O_2^-·、·OH和NO_2^-均有一定的清除作用,且清除效果与多糖浓度呈剂量-效应关系。IC_(50)结果显示湘玉竹多糖清除活性为O_2^-·>DPPH>NO_2^->·OH,而且相同浓度的湘玉竹粗多糖的清除能力强于精制多糖。除了NO_2^-以外,阳性对照VC对DPPH、O_2^-·和·OH自由基的清除能力均强于湘玉竹多糖。结论湘玉竹多糖具有良好的抗氧化能力,可开发成一种新的天然绿色食品抗氧化剂。Objective To explore antioxidative effects of polysaccharides from Polygonatum odoratum in vitro. Methods The polysaccharides were extracted from Polygonatum odoratum using ultrasonic extraction technology and detected by phenol-sulfuric acid method. Compared with the VC positive control, the scavenging activity of crude and refined polysaccharides of Polygonatum odoratum were studied to evaluate their antioxidative effects. Results The polysaccharides contents in crude and refined extracts were 6.05% and 13.26%, respectively. The crude and refined polysaccharides of Polygonatum odoratum had certain scavenging capacity on the DPPH, O2-·, ·OH and NO2-. Within the experimental concentration range, the scavenging effects were in a dose-effect relationship with the concentration. The results of IC50 showed that the order of scavenging activity of Polygonatum odoratum polysaccharides was O2^-·〉DPPH〉NO2^-〉·OH. Furthermore, the scavenging ability of crude polysaccharides was stronger than that of refined polysaccharides in the same concentration. Except for NO2-, the antioxidative effects of VC positive control on DPPH, O2^-· and·OH were stronger than those of the polysaccharides. Conclusion The Polygonatum odoratum polysaccharides have good anti-oxidation ability which can be developed into a new kind of natural green food antioxidant.

关 键 词：湘玉竹 多糖 抗氧化 自由基 清除能力 

分 类 号：TS202.3[轻工技术与工程—食品科学]