基于高通量测序技术的不同性状窖泥微生物组成研究  

Research on microbial composition of pit mud with different traits using high-throughput sequencing

作　　者：罗雯[1] 张倩颖[1] 廖作敏 张文学[1,2] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610000 [2]四川大学锦江学院白酒学院,四川眉山620860

出　　处：《食品与发酵工业》2017年第9期9-14,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(31571824)

摘　　要：借助高通量测序技术,探讨了新泥、趋老熟、老熟、趋老化、老化等不同性状窖泥的微生物群落结构组成。结果显示,趋老熟和老熟窖泥微生物群落的多样性和丰富度均高于其他3种窖泥,且2者的群落分布特征比较相似。趋老熟、老熟窖泥中梭菌属(Clostridium)的优势较为明显,分别为总水平的17.55%和2.99%,而新泥、趋老化泥、老化泥中则以假单胞菌属(Pseudomonas)为绝对优势菌属,占总水平的69.68%、70.50%和72.84%。该研究初步揭示了不同性状窖泥的微生物群落结构差异,为研究判断窖泥性状的微生物组成提供了参考。In order to explore the microbial community structure of pit mud with different traits, microbial compositions of five kinds of pit mud including new pit mud, tending to be aged pit mud, aged pit mud, tending to be aging pit mud and aging pit mud were studied herein by using high-throughput sequencing. The results showed that the distribution characteristics of microbial community in tending to be aged and aged pit muds were similar. Besides, the diversity and abundance of microbial communities in those two pit muds were higher than that in other pit muds. Clostridium was the predominant genera in tending to be aged and aged pit muds, which was 17.55% and 2.99% , respectively. While Pseudomonas was the predominant genera in new, tending to be aging and aging pit muds, accounting for 69.68% , 70.50% and 72.84%. This research preliminarily revealed the microbial community structure of pit mud with different traits, which provided a reference for studying microbial pointers to determine the properties of pit mud.

关 键 词：高通量测序 不同性状 窖泥 微生物组成 

分 类 号：TS261.1[轻工技术与工程—发酵工程]