水不溶性条斑紫菜多糖酸水解的研究  

Investigation of acid hydrolysis of water-insoluble polysaccharide from Porphyra yezoensis

作　　者：王灵昭[1,2] 周晓婉 汪维喜 王纪丰 吕玲玲 舒留泉[1] 姚兴存[1] 吴胜军[1] 焦宇[1] 王玉良 

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室/海洋生命与水产学院,江苏连云港222005 [2]连云港西墅物产有限责任公司,江苏连云港222042

出　　处：《食品与发酵工业》2017年第9期92-96,共5页Food and Fermentation Industries

基　　金：连云港市科技项目(CXY1419);连云港市科技项目(CN1503)

摘　　要：小分子多糖水解产物能够克服大分子多糖溶解性差、生物利用率低的不足。文中对水不溶性条斑紫菜多糖的盐酸水解进行研究。结果表明,盐酸水解多糖的最适条件为,酸浓度0.050 mol/L,温度80℃,时间2.0h。水解产物中还原糖占总糖的0.24%,低聚糖产物可能为二糖或三糖。产物的红外光谱分析表明粉状样品是含硫酸基的β-糖苷键连接的吡喃型低聚糖,其具有糖环内醚结构。产物的紫外光谱分析表明粉状样品含有蛋白质,进一步分析表明样品含有蛋白质6.88%、总糖88.92%。Small molecular weight hydrolysates from polysaccharide hydrolysis are better than big molecular weight polysaccharides on the solubility and bioavailability. In this work, the hydrolysis of water-insoluble polysaccharides from Porphyra yezoensis was investigated using hydrochloric acid method. The optimal conditions for hydrolyzing polysaccharides were determined as： acid concentration 0. 050 mol/L, 80 ℃ reacted for 2.0 hours. In the hydrolysates, the ratio of reducing sugar to total sugar was 0.24% , and the oligosaccharide product could be disaccharide or trisaccharide. The infrared spectrum of powder hydrolysates showed they were pyranoses with sulphuric groups, β-glucosidic bonds and cyclic ether structure. The ultraviolet spectrum of powder hydrolysates showed they contained proteins, and further analysis demonstrated the powder hydrolysates contained 6.88% protein, and 88.92% total sugar.

关 键 词：条斑紫菜 水不溶性多糖 酸水解 红外光谱 紫外光谱 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程]