响应面法优化石榴叶总酚的亚临界水提取工艺  

Optimization of subcritical water extraction for total phenolics from pomegranate( Punica granatum L.) leaves by response surface methodology

作　　者：闫林林[1] 郑光耀[1] 韦朝宽[1] 

机构地区：[1]中国林业科学研究院林产化学工业研究所,国家林业局林产化学工程重点开放性实验室,江苏南京210042

出　　处：《食品工业科技》2017年第19期184-190,共7页Science and Technology of Food Industry

基　　金：中国林业科学研究院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ4)

摘　　要：本研究采用亚临界水萃取法提取石榴叶总酚,并通过响应面法优化其提取工艺参数。在单因素实验的基础上,根据响应面Box-Behnken实验设计原理,采用三因素三水平的分析法,选取提取温度、提取时间、液料比为自变量,考察其对石榴叶提取物中总酚含量的影响,并通过方差分析优化其工艺。结果表明,回归方程对实验拟合较好,可以对石榴叶中总酚含量进行很好的分析和预测;优化后的最佳工艺条件为:提取温度134℃、提取时间31 min、液料比52∶1 m L/g。在该条件下,石榴叶总酚含量为169.24 mg GAE/g,与预测值173.31 mg GAE/g基本一致。通过与溶剂加热回流法相比较,亚临界水提取可明显提高石榴叶总酚的含量,并缩短提取时间。The aim of this study was to optimize the technology parameters of subcritical water extraction for extracting total phenolics from pomegranate leaves by response surface methodology, On the basis of single-factor experiments, three-factors and three-level response surface methodology was applied to investigate the effects of extraction temperature, extraction time and solvent/solid ratio on the total phenolics contents.The results showed that model regression was significant, and the established regression equation for phenolics contents had an excellent of fit.The optimized extraction temperature was 134 ℃, extracting time was 31 minutes,and solvent/solid ratio was 52：1 mL/g.Under the optimized conditions, the total phenolics content was 169.24 mg GAE/g,whicb was consistent with the predictive value of 173.31 nag GAE/g. In addition, the total phenolic content of the optimized extracts was higher than the reflux extraction using aclueous-organic solvent .and the time was shortened.

关 键 词：石榴叶 总酚 亚临界水提取 响应面法 

分 类 号：TS209[轻工技术与工程—食品科学]