豌豆蛋白的提取及利用次豌豆浆发酵乙醇的研究  

Study on the Extraction of Pea Protein and the Fermentation of Ethanol from Sub-pea Pulp

作　　者：邓永东[1] 吕西军 吴学凤[1] 李兴江[1] 

机构地区：[1]合肥工业大学农产品精深加工安徽省重点实验室,安徽合肥230009

出　　处：《农产品加工（下）》2017年第9期1-4,7,共5页Farm Products Processing

基　　金：安徽省重大科技专项"利用次淀粉浆废液发酵酒精资源综合利用的关键技术研究及产业化"(15CZZ03100)

摘　　要：目前,国内利用豌豆提取豌豆蛋白会产生大量废弃液体,这些液体称为次豌豆浆,其中含有大量的豌豆淀粉。一些企业将这部分液体直接排放或者用于生产饲料,造成了资源浪费和环境污染。为了解决这一问题,对豌豆蛋白的提取工艺及其余下的次豌豆浆发酵乙醇展开研究,采用酸沉法对豌豆蛋白进行提取,然后通过试验探究次豌豆浆发酵乙醇的最适条件。结果表明,50 g碗豆中蛋白含量10.84 g,淀粉含量20.34 g,淀粉水解率达到理论值的83.7%;最佳发酵条件为发酵温度30℃,菌液接种量10%,发酵液pH值4,摇床转速200 r/min。在该条件下,乙醇产量达到28.36 g/L,糖利用率达到98%,乙醇产量达到理论值的88%。对次豌豆浆发酵乙醇工艺展开研究将为豌豆综合利用率的提高奠定一定基础。At present,the domestic use of peas to extract pea protein left a large number of abandoned liquid,these liquids are called sub-pea pulp,which contain a lot of pea starch. Some companies will directly discharge this part of the liquid or for the production of feed,resulting in waste of resources and environmental pollution. In order to solve this problem,this paper studies the extraction process of pea protein and the fermentation of fermented ethanol from the other pea pulp. The pea protein is extracted by acid precipitation method,and then the optimum conditions of ethanol fermentation are studied. The results show that 50 g peanut protein content of 10.84 g,starch content of 20.34 g,and the hydrolysis rate of starch reaches 83.7%of the theoretical value. The optimum fermentation conditions are as follows： fermentation temperature 30 ℃, inoculation amount 10%,initial pH 4,shaking speed 200 r/min. Under the optimum conditions,the ethanol yield reaches 28.36 g/L,sugar utilization rate of 98%. Ethanol production reaches 88% of the theoretical value. The research on the fermentation technology of pea pulp is carried out,which las a foundation for the improvement of the comprehensive utilization rate of pea.

关 键 词：次豌豆浆 豌豆蛋白 葡萄糖 乙醇 发酵 

分 类 号：TS201.1[轻工技术与工程—食品科学]