过表达MRP8提高酿酒酵母乙酸耐性及乙醇发酵效率  

Improvement of acetic acid tolerance of Saccharomyces cerevisiae by overexpression of mitochondrial ribosomal protein encoding gene MRP8 for efficient lignocellulosic ethanol production

作　　者：万春 万青青[1] 熊亮[2] 赵心清[1] 白凤武[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室,上海200240 [2]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《生物加工过程》2017年第5期80-85,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21376043;31461143029);国家高技术研究发展计划(863计划)(2012AA021205;2012AA101805)

摘　　要：乙酸是木质纤维素水解液中含量较多的抑制物,因此提高酿酒酵母菌株对乙酸的耐受性有助于提高纤维素乙醇生产效率。本文中,笔者利用基于CRISPR/Cas9系统的基因组编辑技术过表达了酿酒酵母(Saccharomyces cerevisiae)S288c线粒体核糖体蛋白编码基因MRP8,并比较了过表达MRP8的菌株与对照菌株的生长和发酵特性。平板耐性检测发现,MRP8过表达明显提高了菌株的乙酸胁迫耐受性;乙醇发酵结果表明,在4.8 g/L乙酸胁迫条件下,过表达菌株MRP8-3在51 h消耗全部的葡萄糖,发酵时间缩短了25 h,显著优于相同时间的对照菌株。本研究结果为构建高效纤维素乙醇发酵的酿酒酵母菌株提供了新思路。Acet ic acid is one of the major inhibitors derived from the hydrolysis of lignocel lulos ic biomass, and enhancing the tolerance of SaccAaromyces cere^/s/ae to acetic acid is of great importance for improving the lignocel lulosic ethanol product ion.Here,we overexpressed mitochondrial ribosomal protein encoding gene MBPS via CRISPR/Cas9-based genome edit ing in S. cerevisiae S 2 8 8 c,and compared the growth and fermentation performance between the mutants wi th MBPS and the wi ld type.The tolerance to acetic acid was increased by overexpression MBPS on agar plates. Also,mutant MRP8-3 consumed al l glucose wi thin 51 h in the presence of 4. 8 g/L acetic acid,and the fermentation t ime was shortened by 25 h,indicat ing the mutant was superior to the wi ld type.Our f indings provide a novel strategy for constructing robust yeast strains for ef f icient lignocel lulosic ethanol fermentation.

关 键 词：酿酒酵母 MRP8 乙酸胁迫耐受性 乙醇发酵 CRISPR/Cas9 基因组编辑 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]