核酸适配体生物传感技术在食品中重金属铅检测中的应用  

Application of Aptamer Biosensor Technology to Detect Heavy Metal Lead in Food

作　　者：隋佳辰 于寒松[1] 代佳宇 宋战昀[2] 王向辉[1] 郑言[1] 杨倩[1] 张健[1] Sui Jiachen Yu Hansong Dai Jiayu Song Zhanyun Wang Xianghui Zheng Yan Yang Qian Zhang Jianl(Jilin Agricultural University, Changchun 130118 Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062)

机构地区：[1]吉林农业大学,长春130118 [2]吉林出入境检验检疫局,长春130062

出　　处：《中国食品学报》2017年第8期203-209,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31001065);质检总局科技项目(2013IK033)

摘　　要：近年来,我国食品中铅超标事件频发,严重威胁着人民的健康与生命安全。虽然传统的检测技术能对食品中重金属铅进行高选择性和高灵敏度的检测,但是仍需要发展一种简单、快速、准确、成本低廉的方法应用于食品中重金属铅的快速检测。核酸适配体由于其本身具有靶分子广,成本低廉,易体外合成与修饰,操作简单与快捷,分子质量小,特异性高和稳定性好等优点,使核酸适配体生物传感技术在各检测领域具有很强的发展潜质,同时非常适合于食品应急事件中的快速检测。本文简述目前我国食品中重金属铅的污染现状及其来源与危害,对传统检测方法、国家标准检测方法及核酸适配体生物传感技术在食品重金属铅中的检测进展作较详细的论述,并对3种检测方法的优缺点进行评价,最后对核酸适配体生物传感技术在食品重金属铅中的检测前景进行展望。In recent years, lead poisoning cases of food take place in China frequently, which is a serious threat to human health and safety of our people. Although traditional detection techniques can detect lead in food with high selectivity and high sensitivity, but still need to develop a simple, rapid, accurate, and inexpensive method for rapid detection of lead used in food. Because aptamer has wide target molecule, low cost, easy vitro synthesis and modification,simple and quick operation, small molecular weight, high specificity and good stability, so that the aptamer biosensor technology in all detecting areas has strong potential of development, and it is very suitable for food emergencies in the rapid detection. This paper describes the current status quo and their sources of pollution and hazardous for lead in our food, and makes a more detailed summary for progress of detecting lead in the food by the traditional detection methods,the national standard detection methods and aptamer biosensor technology, and evaluates the advantages and disadvantages of the three detection methods, and finally prospects the development of aptamer biosensor technology which detects lead in food.

关 键 词：重金属铅 食品安全 核酸适配体 检测 

分 类 号：TS207.51[轻工技术与工程—食品科学]