垫状卷柏总黄酮的抗氧化和抗肿瘤活性  

Antioxidant and antiproliferative activities of total flavonoids extracted from Selaginella pulvinata(Hook,et Grev.) Maxim

作　　者：郑瑞凤[1] 刘嵬[1] 梁立[1] 杨晨[1] 颜军[1] 苟小军[1] 何钢[1] ZHENG Rui- feng LIU Wei LIANG Li YANG Chen YAN Jun GOU Xiao- Jun HE Gang(Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China)

机构地区：[1]药食同源植物资源开发四川省高校重点实验室四川抗菌素工业研究所成都大学,四川成都610052

出　　处：《食品工业科技》2017年第21期37-41,共5页Science and Technology of Food Industry

基　　金：四川省科技厅应用基础项目(2015JY0117);四川省教育厅基础项目(17ZA0089);四川省高校重点实验室项目(TRCWYXFZCH2016014)

摘　　要：目的:研究垫状卷柏总黄酮的抗氧化能力,以及对人宫颈癌细胞Hela、人乳腺癌细胞MDA-MB-231、人前列腺癌细胞PC-3M的生长抑制作用。方法:采用Fenton法、邻苯三酚自氧化法、DPPH法和还原力测定法评价垫状卷柏总黄酮的体外抗氧化能力。CCK-8法检测垫状卷柏总黄酮的抗肿瘤细胞增殖活性。结果:在总黄酮质量浓度为20~100μg/m L之间,垫状卷柏总黄酮对羟基自由基、超氧阴离子自由基、DPPH自由基的清除率随总黄酮质量浓度升高而增高,半数清除率EC_(50)分别为111.86、89.24、26.51μg/m L;对Hela、PC-3M和MDA-MB-231细胞的抑制作用在总黄酮质量浓度为6.59~79.02μg/m L范围内均呈剂量依赖性,作用时间为72 h的抑制率高于48 h的抑制率。结论:垫状卷柏总黄酮抗氧化活性作用能力强,对Hela、PC-3M和MDA-MB-231细胞具有显著的增殖抑制作用。Objective ：To explore the antioxidant activity of Selaginella pulvinata （Hook, et Grey. ）Maxim of total flavone and its growth inhibitory effect on HeLla, PC-3M and MDA-MB231 cells.Methods： Fenton reaction, pyrogallol auto-oxidation and DPPH radical-scavenging systems were used for antioxidant evaluation of total flavonoids in vitro. The reducing power was evaluated by potassium ferricyanide assay.Growth inhibitory effect of total flavonoids on Hela, PC-3 M and MDA-MB231 cells was assessed by CCK-8 assay.Results：The scavenging effects of the total flavonoids on the hydroxyl, superoxide anion and DPPH free radicals increased as the total flavonoids concentration increased within 20- 100 μg/mL.The EC50 of total flavonoids in scavenging hydroxyl, superoxide anion and DPPH free radicals were 111.86,89.24,26.51 μg/mL, respectively.The growth of HeLa, PC-3M and MDA-MB231 cells was remarkably inhibited by total flavonoids in a dose-dependent manner within the conservation of total flavonoids of 6.59-79.02 μg/mL.The inhibiting Hela,PC-3m and MDA-MB231 cells growth in 72 h was higher than in 48 h. Conclusion ： Total flavonoids of Selaginella pulvinata （ Hook, et Grey. ） Maxim shows strong antioxidant activity and good inhibitory effect against the growth of Hela,PC-3m and MDA-MB231 cells.

关 键 词：垫状卷柏 总黄酮 抗氧化 肿瘤细胞 生长抑制 

分 类 号：TS201.1[轻工技术与工程—食品科学]