紫外诱变选育高产2-苯乙醇酿酒酵母  

Breeding of High Yield 2-phenylethanol Strain from Saccharomyces Cerevisiae by Ultraviolet Mutatgenesis

作　　者：徐峥[1] 褚美芬[1] 张晖[1] 陈毓[1] 

机构地区：[1]杭州医学院,浙江杭州310053

出　　处：《山东化工》2017年第19期50-52,共3页Shandong Chemical Industry

基　　金：杭州医学院自然科学基金(2013XZA02)

摘　　要：2-苯乙醇(2-phenylethanol,PEA)是一种具有玫瑰香气的芳香醇,广泛应用于食品、化妆品和药品等领域。2-苯乙醇对酵母的抑制作用是生物法合成2-苯乙醇的主要瓶颈,本实验通过紫外诱变技术对酿酒酵母AS2.516进行诱变育种,筛选获得了一株2-苯乙醇耐受性和产量均有明显提高的菌株XZ-33,转化合成2-苯乙醇浓度达到3.55 g/L,比原始菌株提高了19.5%,摩尔转化率达到95.7%。2-phenylethanol（2-PEA） as an aromatic alcohol with a rose-like fragrance was widely used in food,cosmetic and pharmaceutical industry.The inhibit ion of 2-phenylethanol to yeast is the main difficult problem in the production of 2-phenylethanol by Saccharomyces cerevisiae.In this study,Saccharomyces cerevisiae AS2.516 was treated by Ultraviolet radiation.As a result,a mutated strain designated XZ-33 was screened out,both tolerance and yield increased significantly,gained the yield of 2-phenylethanol at 3.55 g/L,increased by 19.5% compared with the original strain,the molar conversion rate of L-phenylalanine to 2-phenylethanol is 95.7 %.

关 键 词：酿酒酵母 2-苯乙醇 生物转化 紫外诱变 

分 类 号：TQ926[轻工技术与工程—发酵工程]