纳豆激酶液态发酵生产的研究进展  

Research progress of liquid fermentation of nattokinase

作　　者：吴燕[1,2] 李英波 梁向峰[3] 刘会洲 肖建辉[1] 

机构地区：[1]遵义医学院附属医院,医药生物技术研究所,贵州遵义563003 [2]中国科学院过程工程研究所,中国科学院绿色过程与工程院重点实验室,北京100190 [3]中国科学院青岛生物能源与过程研究所,中国科学院生物基材料重点实验室,山东青岛266101

出　　处：《食品与发酵工业》2017年第10期256-262,共7页Food and Fermentation Industries

基　　金：中国科学院绿色过程与工程实验室青年基金(GPE-2013-3);贵州省高层次创新型人才支撑计划(QKH-RC-20154028);国家自然科学基金(81460156)

摘　　要：纳豆激酶(EC 3.4.21.62)是一种具有强大溶栓活性的丝氨酸蛋白酶。与临床一线溶栓药物(尿激酶和链激酶)相比,纳豆激酶具有安全性好、成本低、半衰期长和可口服等优点,作为溶栓药物或保健食品备受关注。纳豆激酶主要通过固态和液态发酵生产来制备,但现有生产能力偏低,远不能满足市场需求。文中从高产纳豆激酶的菌株选育、培养基优化、发酵条件优化和培养方式等4个方面对近年来相关研究进行了综述,以期为纳豆激酶的生产和应用提供支持。Nattokinase（EC3.4.21.62）is a kind of serine proteinase that has potent thrombolytic activity. Com- pared to the first-line thrombolytic drugs（urokinase and streprokinase） , nattokinase possesses several advantages such as safety, low cost, long half-life and easy oral administration. Therefore, nattokinase has been receiving increasing attention for its use as thrombolytic agent or health care product. The solid and liquid fermentation sate usually em- ployed for the nattokinase production. However, the fermentation titer of nattokinase is still poor, and can meet the re- quirement of the market. In this review, the strain breeding, medium optimization, fermentation conditions optimiza- tion, and culture mode of nattokinase have been outlined and summarized, which would provide valuable reference for the industrial production and application of nattokinase.

关 键 词：纳豆激酶 微生物 液态发酵 溶栓药物 

分 类 号：TQ925[轻工技术与工程—发酵工程]