木瓜蛋白酶辅助提取裙带菜孢子叶多糖的工艺  

Optimization on Papain-assistant Extracting Technological Condition of Polysaccharide from Sporophyll of Undaria pinnatifida

作　　者：熊欣[1,2] 李楠 姜晓野[3] 孙昊 李冬梅 启航 

机构地区：[1]大连工业大学食品学院,大连116034 [2]国家海洋食品工程技术研究中心,大连116034 [3]抚顺市食品检验检测中心,抚顺113000

出　　处：《食品工业》2017年第11期108-113,共6页The Food Industry

基　　金：海洋公益性行业科研专项(编号:201505030-5);国家高技术研究发展计划(863计划;编号:2014AA093602);大连市高层次人才创新支持计划(2016RQ063);辽宁省高等学校优秀科技人才支持计划(LR2015004)

摘　　要：为优化木瓜蛋白酶辅助提取裙带菜孢子叶多糖的工艺,通过单因素试验考察各因素对多糖得率的影响,采用Box-Behnken设计方法建立多糖提取的数学模型。结果表明,单因素的最优条件为:过筛目数120目,浸泡时间12h,浸泡温度50℃。在此前处理基础上,木瓜蛋白酶辅助提取裙带菜孢子叶多糖的最佳工艺条件为:酶解时间6 h,酶加量2.45%,液料比为79∶1(mL/g),裙带菜孢子叶多糖得率7.96%±0.86%。试验结果为裙带菜孢子叶多糖的标准化高效生产提供了一定的理论依据。In order to optimize the process of papain-assistant extracting technology of polysaccharides from sporophyll of Undaria pinnatifida（U.pinnatifida）,effects of various factors on the yield of polysaccharides were investigated by the experiment of single factor.The mathematical model of polysaccharide extraction was established by Box-Behnken design.Results indicated that optimal condition of single factor test was as follows：the mesh number of 120,the soaking time of 12 h and the soaking temperature of 50 ℃.On the basis of this previous treatment,the optimal condition for the extraction of polysaccharides from the sporophyll of U.pinnatifida was as follows：the hydrolysis time of 6 h,the amount of papain of 2.45% and the ratio of water to material of 79∶1（mL/g）,respectively.The yield of polysaccharides extracted from the sporophyll of U.pinnatifida was 7.96%±0.86%.A theoretical basis for the standardized and efficient production of polysaccharides from the sporophyll of U.pinnatifida was provided.

关 键 词：裙带菜 孢子叶 多糖 木瓜蛋白酶 工艺优化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]