UPLC-ELSD法测定12种保健食品中的牛磺酸  

Determination of taurine in health food by ultra-performance liquid chromatography with evaporative light scattering detector

作　　者：康明芹[1] 陈明岩[1] 王岸英[1] 徐立明[1] 张蕊蕊 张旭光[1] 

机构地区：[1]吉林出入境检验检疫局,长春130062 [2]吉林大学公共卫生学院,长春130021

出　　处：《药物分析杂志》2017年第11期1957-1961,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家质检总局科技项目(2012IK162;2013IK163)

摘　　要：目的:建立保健食品中牛磺酸含量的测定方法。方法:采用超高效液相色谱-蒸发光散射(UPLC-ELSD)法测定保健食品中牛磺酸含量。色谱条件:采用BEH Amide(50 mm×2.1 mm,1.7μm)色谱柱,梯度洗脱,流动相A为乙腈,流动相B为0.1%甲酸水,流速0.35m L·min^(-1),柱温:40℃,ELSD漂移管温度40℃,ELSD气体压力150 k Pa,增益值100。结果:牛磺酸浓度在0.10~2.0 mg·m L^(-1)范围内与峰面积呈良好线性关系(r=0.999 9),不同剂型保健食品中牛磺酸的回收率在98.5%~100.7%之间,RSD均小于1.3(n=6)。6批次液体制剂样品的测定结果分别为:38.60、8.702、0.501 0、5.152、5.085、3.821 mg·m L^(-1);3批次固体制剂样品的测定结果分别为:6.703、4.560和15.81 g/100 g;2批次软胶囊样品的测定结果分别为:24.20、8.206 g/100 g;1批硬胶囊样品的测定结果为19.18%。结论:本法经方法学验证,可用于保健食品中牛磺酸的测定。Objective： To establish a method for the determination of the contents of taurine in health food. Method： The content of taurlne in health food was determined by ultra-performance liquid chromatography with evaporative light scattering detector. The chromatographic condition was as follows ： BEH Amide （ 50 mm×2.1 mm, 1.7 μm ） was adopted, the mobile phase A was acetonitrile, the mobile phase B was water containing 0.1% formic acid at the flow rate of 0.35 mL · min-1 and the column temperature was 40℃, the drift tube temperature was 40 ℃, the gas pressure was 20 psi, the yield value was 100. Result： The calibration of taurine concentration was linear in the range of 0.10-2.0 mg· mL-1 （ r=0.999 9 ）, The average contents of taurine in six batches of liquid samples were recovery was 98.5%-100.7% with RSD〈1.3% （ n=6 ）. The 38.60, 8.702, 0.501 0, 5.152, 5.085 and 3.821 mg· mL-1;The contents of taurine in three batches of solid samples were 6.703,4.560 and 15.81 g/100 g; The contents of taurine in two batches of soft capsule samples were 2420 and 8,206 g/100 g; The contents of taurine in one batch of hard capsule sample was 19.18%. Conclusion： It is proved by methodology validation that the method can be used for the determination of the contents of taurine in health food.

关 键 词：牛磺酸 保健食品 超高效液相色谱 蒸发光散射 液体制剂 固体制剂 软胶囊 硬胶囊 

分 类 号：R917[医药卫生—药物分析学]