PCR技术检测食用植物油中猪源成分的应用初探  

Detection of pig derived ingredients in edible oil by PCR technology

作　　者：程铁辕[1] 郑若欣 黄治国[3] CHENG Tie-yuan;ZHENG Ruo-xin;HUANG Zhi-guo(State Key Lab of Liquor Products Test, Sichuan Entry-Exit Inspection and Quarantine Bureau, Yibin 644000, Sichuan, China;Luzhou Product Quality Supervision and Inspection Institute, Luzhou 646000, Sichuan, China;Liquor-making Biotechnology and Application Key Laboratory of Sichuan Province, Sichuan University of Science and Engineering, Zigong 643000, Sichuan, China)

机构地区：[1]四川出入境检验检疫局国家酒类检测重点实验室,四川宜宾644000 [2]泸州市产品质量监督检验所,四川泸州646000 [3]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《粮食与油脂》2017年第11期83-84,共2页Cereals & Oils

摘　　要：采用PCR技术对6种不同来源食用油中的猪源成分进行提取检测,TE缓冲液(pH=8.0)与油脂样品混合定容、涡旋,离心后弃上层油脂,重复操作8~10次后,使用水相提取DNA后用于检测分析。结果表明:市售大豆油和餐馆食用油中猪源成分检测为阴性,市售菜籽油、火锅店食用油、露天夜市摊贩食用油和烧烤店食用油中猪源成分检测为阳性。对照试验和空白试验表明,应用PCR技术检测食用油中猪源性成分是可行的。The application and the feasibility of PCR technology in detection of pig derived ingredients in six kinds of edible oil were studied. TE buffer（pH=8.0） was mixed with the oil sample, and then constant volume and vortex were carried out. Upper layer of oil was discarded after centrifugation. The operation was repeated 8-10 times. DNA was extracted by aqueous phase and then detected. The result showed that pig derived ingredients in the commercially soybean oil and restaurant cooking oil were negative. But pig derived ingredients in the commercial canola oil, edible oil pot shops, open-air night market vendors oil and grill cooking oil were positive. All the results showed that it was feasible to detect pig derived ingredient in edible oil by PCR technology.

关 键 词：聚合酶链式反应 食用油 猪 DNA 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]