矢车菊素-3-葡萄糖苷干预丙烯酰胺细胞毒性的体外模型筛选  

Screening in Vitro Model for Acrylamide-induced Cytotoxicity Intervention by Cyanidin-3-glucoside

作　　者：宋柬 廖小军[1] 胡小松 陈芳 

机构地区：[1]中国农业大学食品科学与营养工程学院国家果蔬加工工程技术研究中心,北京100083 [2]山东省疾病预防控制中心食品与营养所,济南250014

出　　处：《中国食品学报》2017年第9期11-19,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：公益性行业(农业)科研专项(201303073);高等学校基本科研业务费项目(2017QC170);山东省医药卫生科技发展计划项目(2015WS0278)

摘　　要：丙烯酰胺是食品热加工过程中形成的一种内源性化学污染物,能引起细胞毒性。矢车菊素-3-葡萄糖苷作为一种果蔬中广泛存在的花色苷,具有显著的抗氧化活性。目前应用花色苷进行AA细胞毒性的干预尚无系统性研究。为了筛选适用于AA细胞毒性干预的细胞模型,对体外培养的Hep G2、L02、Caco-2、BHK-21及MDA-MB-231等细胞,通过不同浓度AA和Cy-3-glu培养,采用结晶紫染色法测定不同时间的细胞存活率,最终确定AA最适的作用时间为24 h,适宜作用浓度分别为2.5 mmol/L和5.0 mmol/L;Cy-3-glu的最适预处理时间为4 h。筛选出适合Cy-3-glu干预的AA诱导的细胞模型为MDA-MB-231细胞。通过Cy-3-glu抑制细胞内活性氧生成和谷胱甘肽的降低并验证,10~100μmol/L Cy-3-glu预处理表现出显著的AA毒性的保护作用,为毒性干预研究提供模型基础。Acrylamide （AA）, which can cause cytotoxicity, is an endogenous chemical contaminant during food ther- mal processing. Cyanidin-3-glucoside （Cy-3-glu）, as one kind of anthocyanin widely occurring in fruits and vegetables, has obvious antioxidant activity. Recently, there is no systematic study on the intervention against AA cytotoxicity with Cy-3-glu. There were not the systemic studies on the intervention against AA eytotoxicity with Cy-3-glu. The aim of this study is to screen and obtain the suitable cell model to be applied to AA-induced toxicity in vitro. Five cells （HepG2, L02, Caco-2, BHK-21 and MDA-MB-231） were cultured at different AA concentrations and Cy-3-glu. Cell viability was measured using the crystal violet staining and was used as the indicator for cytotoxicity. For AA treatment, the opti- mal incubation duration was 24 h and the suitable concentration was 2.5 mmol/L or 5.0 mmol/L. Moreover, the optimal Cy-3-glu pretreatment time was 4 h. Among five cells, the MDA-MB-231 cells are the most suitable cell model applied for the study on the intervention against AA toxicity with 10-100 μmol/L Cy-3-glu. In addition, Cy-3-glu showed the protective role against reactive oxygen species （ROS） and glutathione （GSH） depletion induced by AA in MDA-MB-231 cells. It would provide a model basis for future research on toxicity intervention.

关 键 词：矢车菊素-3-葡萄糖苷 丙烯酰胺 细胞存活率 体外模型 筛选 

分 类 号：TS201.6[轻工技术与工程—食品科学]