应用碱性过氧化氢法提取黑豆皮可溶性膳食纤维及其胆酸结合能力的研究  

Studies on Extraction of Soluble Dietary Fiber from Black Bean Peel by Alkaline Hydrogen Peroxide and Its Bile Acid Binding Capacity

作　　者：冯子倩 窦唯 刘来玉 胡保明 牛宇戈 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]山西青玉油脂有限公司,太原030032

出　　处：《上海交通大学学报（农业科学版）》2017年第2期89-94,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金青年科学基金项目(31401490);山西青玉油脂有限公司企业资助项目

摘　　要：本文以提高黑豆皮中可溶性膳食纤维提取率及其胆酸结合能力为目的,利用碱性过氧化氢对黑豆皮进行改性,分别研究了H_2O_2浓度、pH、料液比和反应时间对黑豆皮中可溶性膳食纤维提取率的影响;利用正交实验设计L_9(3~4),确定碱性过氧化氢最佳改性条件为H_2O_2浓度15%(V/V),pH 11,料液比1∶18(m/V)及反应时间0.5h。最佳改性条件处理后的黑豆皮可溶性膳食纤维提取率可由原始样品的7.75%提高至16.85%。胆酸结合实验证明改性后的黑豆皮可溶性膳食纤维有着较强的胆酸结合能力,且在低浓度下结合能力更强,说明改性后的黑豆皮膳食纤维具有潜在的降血脂功能,有望被开发成一种预防心血管疾病的食品添加剂。The study was to increase the extraction yield of soluble dietary fibers(SDF)from black bean peel,as well as improve the bile acid binding capacity of SDF by alkaline hydrogen peroxide.The influence of concentrations of H_2O_2,pH value,liquid-to-solid ratio and reaction time on the extraction yield of SDF were determined respectively followed by the L_9(3~4)orthogonal test.The results showed that the optimal modification conditions were as below:15% H_2O_2(V/V),pH 11,liquid-to-solid ratio as 1∶18(m/V)and reaction time as 0.5h,under which the extraction yield of SDF increased significantly from 7.75% to 16.85%.In addition,the results of bile acid binding capacity assay demonstrated modified SDF had great abilities of binding bile acids,especially at a lower concentration.It indicated that modified SDF had a great potential of reducing the plasma lipid levels and could be developed as a novel food additive to prevent cardiovascular disease.

关 键 词：黑豆皮 可溶性膳食纤维 改性 胆酸结合 

分 类 号：TS201.2[轻工技术与工程—食品科学]