基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究  

Study on the Chemical Constituents of Pu-erh Teas from Different Areas by UHPLC-Q-TOF/MS

作　　者：杨晨 戴伟东 吕美玲[4] 李朋亮[1,2,3] 刘栩 田军[5] 万云龙 李继[5] 林智 

机构地区：[1]农业部茶树生物学与资源利用重点实验室 [2]中国农业科学院茶叶研究所,浙江杭州310008 [3]中国农业科学院研究生院,北京100081 [4]安捷伦科技(中国)有限公司,北京100102 [5]昆明七彩云南庆沣祥茶业股份有限公司,云南昆明650501

出　　处：《茶叶科学》2017年第6期605-615,共11页Journal of Tea Science

基　　金：国家自然科学基金(31500561);中央级公益性科研院所所级基本科研业务费专项(1610212016008);现代农业产业技术体系建设专项资金(CARS-23)

摘　　要：为了更加全面地了解产地对普洱生茶品质与化学成分的影响,本研究选取来自临沧市、普洱市、西双版纳州三大产区12个茶山(自然村)的普洱生茶样品,采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q-TOF/MS)对不同产地普洱生茶的非挥发性代谢物表型进行了研究。结果表明:不同产地普洱生茶的化学成分在含量上具有较大差异,且具有明显的产地特征。采用主成分分析,可以对来自西双版纳自治州(包括勐腊县、勐海县、景洪市)、普洱市、临沧市3个地级行政区的普洱生茶进行有效区分,也可以对来自普洱茶产区的东南、西南、西北3个区域的普洱生茶进行有效区分。进一步鉴定了普洱生茶中79种主要成分,并对其在12个茶山(自然村)的普洱生茶中的含量分布,以及与不同产地普洱生茶滋味品质的关系进行了分析。本研究表明,基于UHPLC-Q-TOF/MS的茶叶非挥发性化学成分轮廓可以作为普洱生茶产地判别的依据。In order to fully understand the influences of the production area on pu-erh raw tea quality and chemical composition, 12 pu-erh raw tea samples were selected from different mountains （villages） in Lincang, Pu＇er, and Xishuangbanna. The nonvolatile tea metabolite phenotypes of the pu-erh raw teas were analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry-four （UHPLC-Q-TOF/MS）. The results showed that the pu-erh raw teas from different areas had distinct chemical compositions. Pu-erh raw teas from Xishuangbanna （including Mengla, Menghai, and Jinghong）, Pu＇er, and Lincang could be seperated by principal component analysis. Pu-erh raw teas could also be seperated according to producing areas （southeast, southwest, and northwest of pu-erh tea area）. Seventy nine main components in pu-erh raw teas were identified, and the relationships between the components and the taste qualities of the 12 pu-erh raw teas were also analyzed. This study showed that the metabolite phenotypes analyzed by UHPLC-Q-TOF/MS could be used for the discrimination of pu-erh raw teas according to producing areas.

关 键 词：普洱生茶 产地 化学成分 液质联用 代谢组学 滋味 

分 类 号：O657.63[理学—分析化学] TS272.7[理学—化学]