菊芋渣选择性吸附天然菊粉酶催化制备短链低聚果糖  

Selective Adsorption of Native Inulinase by Jerusalem Artichoke Residue and Preparation of Short-chain Fructooligosaccharides

作　　者：李鑫[1,2] 周瑾[2] 赖晨欢[1,2] 勇强[1,2] 

机构地区：[1]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [2]南京林业大学化学工程学院,江苏南京210037

出　　处：《生物质化学工程》2017年第6期33-37,共5页Biomass Chemical Engineering

基　　金：江苏省重点研发计划项目(BF2015007);青海省重点研发与转化计划项目(2016-HZ-819);江苏省高校优势学科建设工程资助项目(无编号)

摘　　要：以菊粉加工废弃物(菊芋渣)选择性吸附黑曲霉产的天然菊粉酶提高菊粉酶活力(I)与转化酶活力(S)的比值(即I/S比值),并将此纯化后的菊粉酶应用于后续的短链低聚果糖(FOSs)的生产,考察了纯化后菊粉酶对FOSs产率的影响。结果显示:选择的较佳吸附条件为:菊芋渣质量浓度为100 g/L,吸附p H值为5.0,吸附温度为4℃。经菊芋渣的选择性吸附,天然菊粉酶酶系中转化酶活力降低80.84%,菊粉酶活力保留67.92%,I/S比值由原来的1.13上升至4.02。经菊芋渣选择性吸附后的天然菊粉酶用于制备短链低聚果糖,可显著提高短链低聚果糖的浓度,酶解条件为:菊粉质量浓度为40 g/L,酶解p H值为5.0,温度为50℃,酶用量为20 U/g(以菊粉质量计),短链低聚果糖质量浓度达到16.06g/L,是原酶液制备短链低聚果糖的2.55倍;短链低聚果糖得率为40.16%。A selective adsorption method was developed to adsorb native inulinase from Aspergillus niger to reduce sucrase activity by Jerusalem artichoke residue. The inulinase activity（ I）/sucrase activity（ S） ratio was generally used to characterize the inulinases. Afterward,the native inulinase,selectively adsorbed by Jerusalem artichoke residue,was applied for production of short-chain fructooligosaccharides（ FOSs）. The results showed that the optimal conditions of the selective adsorption were as follows： Jerusalem artichoke residue loading 100 g/L,p H value 5. 0,adsorption temperature 4 ℃. 67. 92% Inulinase activity was retained and 80. 84% sucrase activity was decreased in the native inulinase system after the selective adsorption. The I/S ratio increased from 1. 13 to 4. 02. The selective adsorption of native inulinase by Jerusalem artichoke residue improved the production of short-chain FOSs. The conditions of enzymatic hydrolysis were inulin 40 g/L,p H value 5. 0,50 ℃,inulinase loading 20 U/g（ based on the mass of inulin）. The mass concentration of short-chain FOSs was 16. 06 g/L,which was 2. 55 times than that of the original inulinase. The yield of short-chain FOSs was 40. 16%.

关 键 词：菊粉酶 选择性吸附 菊芋渣 菊粉 短链低聚果糖 

分 类 号：O636.1[理学—高分子化学]