产纤溶酶乳酸菌筛选及安全性评估  

Screening and safety evaluation of lactic acid bacteria producing fibrinolytic enzyme

作　　者：尹梦雯 周倩 雷婵 胡欣洁[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品与机械》2017年第10期28-33,共6页Food and Machinery

摘　　要：以从传统发酵食品中分离出的2株产纤溶酶的粪肠球菌HYD09与HYN06为对象,测定其发酵上清液酶活,对菌株进行安全性评估。选较安全的菌株进行胃肠道生存能力测试;同时对发酵上清液进行蛋白酶处理试验;从发酵液中获得粗酶液,进行活性电泳。结果表明:HYD09和HYN06发酵上清液酶活分别为115.3 U/mL和107.2 U/mL;HYD09和HYN06吲哚试验、硝酸盐还原酶试验、氨基脱羧酶试验、溶血试验均为阴性,对14种抗生素无多重耐药性;HYD09无毒力基因检出,HYN06检出esp毒力基因。证明HYD09菌株是安全的。进一步研究发现,菌株HYD09有一定的耐酸耐胆盐能力;HYD09发酵上清液经胃蛋白酶和胰蛋白酶处理仍具有纤溶活性;且菌株HYD09发酵上清液的粗酶中有2种主要的组分,但具有纤溶活性的组分只有1种。In this study, 2 strains of Enterococcus faecalis HYD09 and HYNO6 isolated from traditional fermented food were used to determine lhe enzyme activity of fermentation supernatant, and evaluate the basic safety of the strains. The safer strain were tested for gastrointestinal viability; meanwhile, the fermentation supernatant was subjected to protease; the crude enzyme was obtained from the fermentation broth and its activity was measured by Native-Page. The results showed： the enzyme activities of HYD09 and HYN06 fermentation supernatant were 115. 3 U/mL and 107.2 U/mL, respeetively; HYD09 and HYN06 indole test, nitrate reductase assay, amino acid decarboxylase test hemolysis test were negative, no multiple antibiotic resistance to 14 kinds of antibiotics. Moreover, no virulence gene was detected in strain HYD09, while the virulence gene esp was detected in strain HYN06. This confirmed that HYD09 strains were safe. Further studies showed that the HYD09 had the ability of acid resistance and bile salt tolerance; besides, the fermentation supernatant of strain HYD09 still had fibrinolytie activity after pepsin and trypsin treatment, Two main corn ponents were found in the crude enzyme of the fermentation supernatant of HYD09 strain, and one of them had fibrinolytic activity.

关 键 词：纤溶酶 乳酸菌 鉴定 安全性 活性电泳 

分 类 号：TS201.3[轻工技术与工程—食品科学]