摄食DHA-磷脂酰丝氨酸对发育期小鼠体组织DHA水平的影响  

Effect of Dietary DHA-Phosphatidylserine on DHA Level of Body Tissues in Developing Mice

作　　者：吴芳[1] 王丹丹[1] 温敏[1] 薛长湖[1] 王玉明[1] WU Fang;WANG Dandan;WEN Min;XUE Changhu;WANG Yuming(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Chin)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2018年第1期131-135,共5页Food Science

基　　金：国家自然科学基金面上项目(31371757);国家自然科学基金重点项目(31330060)

摘　　要：目的:比较研究断乳后干预二十二碳六烯酸-磷脂酰丝氨酸(docosahexaenoic acid-phosphatidylserine,DHA-PS)和DHA-甘油三酯(DHA-triglyceride,DHA-TG)对发育期ICR小鼠体组织DHA含量以及脂肪酸组成的影响。方法:母鼠孕期及哺乳期喂饲n-3多不饱和脂肪酸(n-3 polyunsaturated fatty acids,n-3 PUFAs)缺乏饲料,子代雄小鼠断乳后随机分为3组,分别喂饲n-3 PUFAs缺乏饲料(n-3缺乏组)、DHA-TG饲料(DHA-TG组)、DHA-PS饲料(DHA-PS组),每组8只,喂养2周后检测小鼠脑皮质、精巢、肝脏、红细胞中脂肪酸组成和DHA含量。结果:补充DHA-PS和DHA-TG可以使发育期小鼠各组织中DHA含量显著增加(P<0.05),二十二碳五烯酸和花生四烯酸含量显著降低(P<0.05)。DHA-TG组精巢中DHA含量明显高于DHA-PS组;DHA-PS组肝脏TG、磷脂和红细胞中DHA水平显著高于DHA-TG组(P<0.05),而两组脑皮质DHA水平无显著性差异(P>0.05)。结论:膳食补充DHA-PS和DHA-TG均可明显提高发育期小鼠各组织中DHA水平,但二者的组织蓄积特性不同,DHA-PS可以更高效地提高小鼠肝脏和红细胞中DHA水平。Objective： To investigate the comparative effects of post-weaning dietary docosahexaenoic acid-phosphatidylserine （DHA-PS） and DHA-triglyceride （DHA-TG） on DHA concentration and fatty acid composition of lipids in various tissues of developing mice. Methods： ICR female mice were fed n-3 polyunsaturated fatty acids （PUFAs） defcient diet during maternal pregnancy and lactation, and the weanling （3-week old） ICR-strain male mouse pups were randomly assigned to three groups, which were fed three different types of diets including n-3 PUFAs defciency （n-3 PUFAs Def group）, DHA-TG （DHA-TG group） and DHA-PS （DHA-PS group）, respectively. After 2 weeks of feeding, all mouse pups were sacrifced, and cortex, testis, liver, and red blood cells were harvested for detecting DHA level and fatty acid composition of lipids. Results： Compared to the n-3 Def group, the concentration of DHA in developing mouse tissues in the DHA-TG and DHA-PS groups was signifcantly increased, whereas the concentrations of docosapentaenoic acid （DPA） and arachidonic acid （AA） were decreased. The DHA-PS group exhibited signifcantly higher DHA levels in erythrocytes, liver triglyceride and phospholipid but lower DHA level in testis compared with the DHA-TG group. There was no difference in DHA level in cortex between the two groups. Conclusion： Dietary supplementation of DHA-PS and DHA-TG signifcantly increased the level of DHA in the tissues of developing mice, but resulted in different accumulation patterns of DHA and DHA-PS more effectively increased DHA contents in the liver and erythrocytes.

关 键 词：二十二碳六烯酸 二十二碳六烯酸-磷脂酰丝氨酸 甘油三酯 磷脂 小鼠 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]