吸水链霉菌多糖的纯化鉴定及其性质  

Purification,Identification and Property of Polysaccharides From Streptomyces hygroscopicus BS-112

作　　者：张楠 李武[1] 彭慧娟 段辉国 张志勇 

机构地区：[1]四川省高等学校特色农业资源研究与利用重点实验室,四川内江641100 [2]山东农业大学生命科学学院,山东泰安271018

出　　处：《核农学报》2017年第12期2367-2376,共10页Journal of Nuclear Agricultural Sciences

基　　金：四川省教育厅重点项目(11ZA029);川西珍稀植物资源研究与利用创新团队项目(14TD0025)

摘　　要：为探讨从吸水链霉菌BS-112发酵液中分离的一种新多糖的生物学特性,采用凝胶过滤层析、离子交换层析及高效凝胶渗透色谱(HPGPC)对吸水链霉菌多糖(SHP)进行分离纯化,利用HPGPC、HPLC和红外光谱分析SHP纯品的部分结构特征,通过微量稀释法测定SHP对食源性致病菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并开展SHP对小鼠的急性毒性试验。结果表明,SHP是由单一葡萄糖残基组成的中性胞外多糖,相对分子质量约为2.3×10~3。SHP对大肠杆菌、金黄色葡萄球菌、沙门氏菌、李斯特菌、枯草芽孢杆菌的MIC分别为15.63、31.25、15.63、31.25、31.25μg·m L^(-1),MBC分别为31.25、62.50、31.25、125.00、62.50μg·m L^(-1)。SHP对小鼠经口灌胃的LD50>5 000 mg·kg-1。综上,SHP对常见食源性致病细菌有良好的抑制作用且无毒,具有作为食品防腐保鲜剂的发展前景。To explore the biological property of a novel polysaccharide （SHP for short） isolated from Streptomyces hygroscopicus BS- 112 （SHP for short）, SHP was extracted and purified using gel filtration chromatograph and ion exchange chromatography respectively, and then purified them by preparative high-performance gel permeation chromatography （HPGPC）. The partial chemical structure of SHP was identified using HPGPC, HPLC and FT - IR. The results showed that SHP was a kind of low molecular weight homopolysaccharide composed of β - D-glucopyranose and acetyl groups. The relative molecular mass of antibacterial polysaccharides is 2.3 × 10^3. The minimum inhibitory concentration （MIC）and minimum bactericidal concentration （MBC） against food-borne bacterial pathogens were determined by the microdilution broth method. The MIC of SHP against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes and Bacillus subtilis were 15.63, 31.25, 15.63, 31.25 and 31.25 μg·mL^-1, respectively, and their MBC were 31.25, 62.50, 31.25, 125.00 and 62.50 μg·mL^-1 , respectively. Moreover, the acute toxicity of SHP on mice was also assessed by standard method. LDso of SHP to Kunming mice by intragastric administration was over 5 000 mg· kg^-1 , belonging to substance of actually non-toxic grade. In summary, SHP showed strong inhibitory against common food-borne pathogens, which are actually non-toxic level substances with good thermal stability and broad pH tolerance, suggesting that SHP might be a potential food preservative.

关 键 词：吸水链霉菌多糖 抑菌活性 分离纯化 鉴定 生物学性质 

分 类 号：TS202.3[轻工技术与工程—食品科学]